ARG24125
anti-SHANK3 antibody [S69]
anti-SHANK3 antibody [S69] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Overview
| Product Description | Mouse Monoclonal antibody [S69] recognizes SHANK3 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | S69 |
| Isotype | IgG2b |
| Target Name | SHANK3 |
| Antigen Species | Rat |
| Immunogen | Synthetic peptide of Rat Shank3 |
| Conjugation | Un-conjugated |
| Protein Full Name | SH3 and multiple ankyrin repeat domains protein 3 |
| Alternate Names | SHANK3; SH3 And Multiple Ankyrin Repeat Domains 3; PSAP2; KIAA1650; SPANK-2; PROSAP2; SH3 And Multiple Ankyrin Repeat Domains Protein 3; Proline Rich Synapse Associated Protein 2; Shank3 Postsynaptic Density Protein; Proline-Rich Synapse-Associated Protein 2; Shank Postsynaptic Density Protein; DEL22q13.3; Prosap2; ProSAP2; SCZD15; Shank3 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 50% Glycerol and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SHANK3 |
| Gene Full Name | SH3 And Multiple Ankyrin Repeat Domains 3 |
| Function | his gene is a member of the Shank gene family. Shank proteins are multidomain scaffold proteins of the postsynaptic density that connect neurotransmitter receptors, ion channels, and other membrane proteins to the actin cytoskeleton and G-protein-coupled signaling pathways. Shank proteins also play a role in synapse formation and dendritic spine maturation. Mutations in this gene are a cause of autism spectrum disorder (ASD), which is characterized by impairments in social interaction and communication, and restricted behavioral patterns and interests. Mutations in this gene also cause schizophrenia type 15, and are a major causative factor in the neurological symptoms of 22q13.3 deletion syndrome, which is also known as Phelan-McDermid syndrome. Additional isoforms have been described for this gene but they have not yet been experimentally verified. |
| Cellular Localization | Cell projection, Cytoplasm, Synapse |
| Highlight | Major scaffold postsynaptic density protein which interacts with multiple proteins and complexes to orchestrate the dendritic spine and synapse formation, maturation and maintenance. Interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and HOMER, respectively, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction through the interaction with Arp2/3 and WAVE1 complex as well as the promotion of the F-actin clusters. By way of this control of actin dynamics, participates in the regulation of developing neurons growth cone motility and the NMDA receptor-signaling. Also modulates GRIA1 exocytosis and GRM5/MGLUR5 expression and signaling to control the AMPA and metabotropic glutamate receptor-mediated synaptic transmission and plasticity. May be required at an early stage of synapse formation and be inhibited by IGF1 to promote synapse maturation. |
| Calculated MW | 184 kDa |
| PTM | Methylation, Phosphoprotein |
Images (3) Click the Picture to Zoom In
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ARG24125 anti-SHANK3 antibody [S69] IHC-P image
Immunohistochemistry: Mouse Backskin stained with ARG24125 anti-SHANK3 antibody [S69] at 1:100 dilution.
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ARG24125 anti-SHANK3 antibody [S69] ICC/IF image
Immunofluorescence: SH-SY5Y stained with ARG24125 anti-SHANK3 antibody [S69] at 1:50 dilution.
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ARG24125 anti-SHANK3 antibody [S69] WB image
Western blot: Rat brain stained with ARG24125 anti-SHANK3 antibody [S69] at 1:1000 dilution.
