ARG44208

anti-SF20 antibody

anti-SF20 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes SF20
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name SF20
Antigen Species Human
Immunogen Recombinant protein of Human SF20
Conjugation Un-conjugated
Protein Full Name Myeloid-derived growth factor
Alternate Names MYDGF; Myeloid Derived Growth Factor; R33729_1; IL25; SF20; Interleukin 27 Working Designation; Myeloid-Derived Growth Factor; C19orf10; IL-25; IL-27; IL27; Stromal Cell-Derived Growth Factor SF20; Chromosome 19 Open Reading Frame 10; UPF0556 Protein C19orf10; EUROIMAGE1875335; Interleukin-25; C19ORF10; IL27w; IL27W

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1-3 µg/1x10^6 cells
IHC-P2-5 µg/ml
WB0.1-0.25 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA.
Preservative 0.05% Sodium azide
Stabilizer 5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 28106 Mouse MYDGF

GeneID: 56005 Human MYDGF

Swiss-port # Q969H8 Human Myeloid-derived growth factor

Swiss-port # Q9CPT4 Mouse Myeloid-derived growth factor

Gene Symbol MYDGF
Gene Full Name Myeloid Derived Growth Factor
Background The protein encoded by this gene was previously thought to support proliferation of lymphoid cells and was considered an interleukin. However, this activity has not been reproducible and the function of this protein is currently unknown.
Function Bone marrow-derived monocyte and paracrine-acting protein that promotes cardiac myocyte survival and adaptive angiogenesis for cardiac protection and/or repair after myocardial infarction (MI). Stimulates endothelial cell proliferation through a MAPK1/3-, STAT3- and CCND1-mediated signaling pathway. Inhibits cardiac myocyte apoptosis in a PI3K/AKT-dependent signaling pathway.
Cellular Localization Endoplasmic reticulum, Golgi apparatus, Secreted
Calculated MW 19 kDa

Images (6) Click the Picture to Zoom In

  • ARG44208 anti-SF20 antibody IHC-P image

    Immunohistochemistry: Human colorectal adenocarcinoma stained with ARG44208 anti-SF20 antibody at 2 μg/mL dilution.

  • ARG44208 anti-SF20 antibody WB image

    Western blot: HepG2, PC-3, U251 and THP-1 stained with ARG44208 anti-SF20 antibody at 0.25 μg/mL dilution.

  • ARG44208 anti-SF20 antibody FACS image

    Flow Cytometry: JK stained with ARG44208 anti-SF20 antibody at 1 μg/1x10^6 cells dilution.

  • ARG44208 anti-SF20 antibody WB image

    Western blot: Rat liver stained with ARG44208 anti-SF20 antibody at 0.25 μg/mL dilution.

  • ARG44208 anti-SF20 antibody WB image

    Western blot: Mouse stomach and Mouse liver stained with ARG44208 anti-SF20 antibody at 0.25 μg/mL dilution.

  • ARG44208 anti-SF20 antibody IHC-P image

    Immunohistochemistry: Human lung cancer stained with ARG44208 anti-SF20 antibody at 2 μg/mL dilution.