ARG67046
anti-SF1 antibody [SQab30309]
anti-SF1 antibody [SQab30309] for IHC-Formalin-fixed paraffin-embedded sections and Human
Overview
| Product Description | Recombinant rabbit Monoclonal antibody [SQab30309] recognizes SF1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab30309 |
| Isotype | IgG |
| Target Name | SF1 |
| Antigen Species | Human |
| Immunogen | Recombinant protein of Human SF1. |
| Conjugation | Un-conjugated |
| Protein Full Name | Steroidogenic factor 1 |
| Alternate Names | SF1, Splicing Factor 1, ZFM1, Zinc Finger Protein 162, ZCCHC25, ZNF162, Mammalian Branch Point-Binding Protein, Zinc Finger Gene In MEN1 Locus, Transcription Factor ZFM1, MBBP, BBP, D11S636 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | Adrenocortical adenoma |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05%BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05%BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SF1 |
| Gene Full Name | Splicing Factor 1 |
| Background | This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016] |
| Function | Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor. [UniProt] |
| Cellular Localization | Nucleus; Spliceosome |
| Calculated MW | 68 kDa |
| PTM | Phosphorylation on Ser-20 interferes with U2AF2 binding and spliceosome assembly. Isoform 6 is phosphorylated on Ser-463. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG67046 anti-SF1 antibody [SQab30309] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded adrenocortical adenoma stained with adrenocortical adenoma ARG67046 anti-SF1 antibody [SQab30309].
