ARG63166
anti-SET / I2 alpha PP2A antibody
anti-SET / I2 alpha PP2A antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Immune System antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes SET / I2 alpha PP2A |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Cow |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognize isoform 2 (NP_003002.2). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SET / I2 alpha PP2A |
| Antigen Species | Human |
| Immunogen | SAPAAKVSKKELNS-C |
| Conjugation | Un-conjugated |
| Alternate Names | I-2PP2A; Template-activating factor I; I2PP2A; Inhibitor of granzyme A-activated DNase; Protein SET; TAF-IBETA; IPP2A2; TAF-I; PHAPII; 2PP2A; Phosphatase 2A inhibitor I2PP2A; IGAAD; HLA-DR-associated protein II |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | The protein encoded by this gene inhibits acetylation of nucleosomes, especially histone H4, by histone acetylases (HAT). This inhibition is most likely accomplished by masking histone lysines from being acetylated, and the consequence is to silence HAT-dependent transcription. The encoded protein is part of a complex localized to the endoplasmic reticulum but is found in the nucleus and inhibits apoptosis following attack by cytotoxic T lymphocytes. This protein can also enhance DNA replication of the adenovirus genome. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Immune System antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 33 kDa |
| PTM | Isoform 2 is phosphorylated on Ser-15 and Ser-24. Isoform 2 is acetylated on Lys-11. Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group (By similarity). N-terminus of isoform 1 is methylated by METTL11A/NTM1. Mainly trimethylated (By similarity). Isoform 2 is cleaved after Lys-176 by GZMA. The cleavage inhibits its nucelosome assembly activity and disrupts the inhibition on NME1. |
Images (4) Click the Picture to Zoom In
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ARG63166 anti-SET / I2 alpha PP2A antibody WB image
Western Blot: Human Kidney lysate (35 µg protein in RIPA buffer) stained with ARG63166 anti-SET / I2 alpha PP2A antibody at 0.3 µg/ml dilution.
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ARG63166 anti-SET / I2 alpha PP2A antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human adrenal gland tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63166 anti-SET / I2 alpha PP2A antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG63166 anti-SET / I2 alpha PP2A antibody WB image
Western blot: 35 µg of Daudi (A) and MOLT-4 (B) cell lysates (in RIPA buffer) stained with ARG63166 anti-SET / I2 alpha PP2A antibody at 0.01 µg/ml dilution and incubated at RT for 1 hour.
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ARG63166 anti-SET / I2 alpha PP2A antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human testis tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63166 anti-SET / I2 alpha PP2A antibody at 3.75 µg/ml dilution followed by AP-staining.
