ARG58622
anti-SERPINB1 antibody
anti-SERPINB1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Goat Polyclonal antibody recognizes SERPINB1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SERPINB1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide from the internal region of Human SERPINB1 (NP_109591.1). (C-KTINQWVKGQTEGK) |
| Conjugation | Un-conjugated |
| Alternate Names | EI; LEI; ELANH2; Monocyte/neutrophil elastase inhibitor; Leukocyte elastase inhibitor; M/NEI; Serpin B1; MNEI; PI-2; PI2; Peptidase inhibitor 2; HEL57 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 42 - 45 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Ammonium sulphate precipitation followed by affinity purification with immunogen. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SERPINB1 |
| Gene Full Name | serpin peptidase inhibitor, clade B (ovalbumin), member 1 |
| Background | The protein encoded by this gene is a member of the serpin family of proteinase inhibitors. Members of this family maintain homeostasis by neutralizing overexpressed proteinase activity through their function as suicide substrates. This protein inhibits the neutrophil-derived proteinases neutrophil elastase, cathepsin G, and proteinase-3 and thus protects tissues from damage at inflammatory sites. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012] |
| Function | Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3. Also functions as a potent intracellular inhibitor of granzyme H. [UniProt] |
| Calculated MW | 43 kDa |
Images (2) Click the Picture to Zoom In
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ARG58622 anti-SERPINB1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human spleen tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG58622 anti-SERPINB1 antibody at 5 µg/ml dilution followed by AP-staining.
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ARG58622 anti-SERPINB1 antibody WB image
Western blot: 35 µg of Human placenta lysate (in RIPA buffer) without blocking peptide (-) or with blocking peptide (+). The blots were stained with ARG58622 anti-SERPINB1 antibody at 0.5 µg/ml dilution. Primary incubation was 1 hour. Detected by chemiluminescence.
