ARG10653
anti-SERCA2 ATPase antibody
anti-SERCA2 ATPase antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes SERCA2 ATPase |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SERCA2 ATPase |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the center region of Human SERCA2 ATPase. 100% homologous in Human, Mouse and Rat. (QRDACLNARCFARVE) |
| Conjugation | Un-conjugated |
| Alternate Names | ATP2B; Endoplasmic reticulum class 1/2 Ca; SERCA2; SR Ca; DD; Sarcoplasmic/endoplasmic reticulum calcium ATPase 2; EC 3.6.3.8; Calcium pump 2; DAR; Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform; 2+ |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 0.025% Sodium azide and 2.5% BSA. |
| Preservative | 0.025% Sodium azide |
| Stabilizer | 2.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | ATP2A2 |
| Gene Full Name | ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 |
| Background | This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2008] |
| Function | This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Isoform 2 is involved in the regulation of the contraction/relaxation cycle. [UniProt] |
| Calculated MW | 115 kDa |
| PTM | Nitrated under oxidative stress. Nitration on the two tyrosine residues inhibits catalytic activity. |
Images (2) Click the Picture to Zoom In
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ARG10653 anti-SERCA2 ATPase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast cancer tissue stained with ARG10653 anti-SERCA2 ATPase antibody.
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ARG10653 anti-SERCA2 ATPase antibody WB image
Western blot: 1) Rat skeletal muscle, 2) Rat kidney, 3) Human PANC, and 4) SMMC-7721 cell lysates stained with ARG10653 anti-SERCA2 ATPase antibody.
