ARG64746
anti-SEPT7 antibody
anti-SEPT7 antibody for Western blot and Human
Cell Biology and Cellular Response antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes SEPT7 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog, Hrs, Pig |
| Tested Application | WB |
| Specificity | This antibody is expected to recognize all three reported isoforms (NP_001779.3; NP_001011553.2; NP_001229885.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SEPT7 |
| Antigen Species | Human |
| Immunogen | C-DNNKNKGQLTKSP |
| Conjugation | Un-conjugated |
| Alternate Names | SEPTIN7; Septin 7; Septin-7; SEPT7A; CDC10; SEPT7; CDC3; CDC10 Protein Homolog; CDC10 (Cell Division Cycle 10, S. Cerevisiae, Homolog); CDC10 Cell Division Cycle 10 Homolog (S. Cerevisiae); NBLA02942 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SEPTIN7 |
| Gene Full Name | septin 7 |
| Background | This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19. [provided by RefSeq, Jul 2011] |
| Function | Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Required for normal progress through mitosis. Involved in cytokinesis. Required for normal association of CENPE with the kinetochore. Plays a role in ciliogenesis and collective cell movements. Forms a filamentous structure with SEPTIN12, SEPTIN6, SEPTIN2 and probably SEPTIN4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation (PubMed:25588830). [Uniprot] |
| Cellular Localization | Cell projection, Centromere, Chromosome, Cilium, Cytoplasm, Cytoskeleton, Flagellum, Kinetochore. [Uniprot] |
| Research Area | Cell Biology and Cellular Response antibody |
| Calculated MW | 51 kDa |
| PTM | Acetylation, Phosphoprotein. [Uniprot] |
Images (1) Click the Picture to Zoom In
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ARG64746 anti-SEPT7 antibody WB image
Western blot: 35 µg of nuclear HeLa lysate (in RIPA buffer) stained with ARG64746 anti-SEPT7 antibody at 0.5 µg/ml dilution and incubated at RT for 1 hour.
