ARG51672
anti-SEK1 / MKK4 phospho (Thr261) antibody
anti-SEK1 / MKK4 phospho (Thr261) antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat
Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes SEK1 / MKK4 phospho (Thr261) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SEK1 / MKK4 |
| Antigen Species | Human |
| Immunogen | Peptide sequence around phosphorylation site of threonine261 (A-K-T(p)-RD) derived from Human SEK1/MKK4. |
| Conjugation | Un-conjugated |
| Alternate Names | MEK 4; MAPK/ERK kinase 4; PRKMK4; SAPKK-1; SAPK/ERK kinase 1; SKK1; JNK-activating kinase 1; EC 2.7.12.2; MEK4; MAP kinase kinase 4; c-Jun N-terminal kinase kinase 1; SEK1; SAPKK1; MAPKK4; Stress-activated protein kinase kinase 1; JNKK1; MKK4; SERK1; SAPK kinase 1; Dual specificity mitogen-activated protein kinase kinase 4; JNKK; MAPKK 4 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Buffer | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P45985 Human Dual specificity mitogen-activated protein kinase kinase 4 Swiss-port # P47809 Mouse Dual specificity mitogen-activated protein kinase kinase 4 |
|---|---|
| Gene Symbol | MAP2K4 |
| Gene Full Name | mitogen-activated protein kinase kinase 4 |
| Background | Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1). |
| Function | Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 seems to be the prerequisite for JNK activation at least in response to proinflammatory cytokines, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs. MAP2K4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Whereas MAP2K7/MKK7 exclusively activates JNKs, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAPK12, MAPK13 and MAPK14. [UniProt] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 44 kDa |
| PTM | Activated by phosphorylation on Ser-257 and Thr-261 by MAP kinase kinase kinases (MAP3Ks). |
Images (2) Click the Picture to Zoom In
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ARG51672 anti-SEK1 / MKK4 phospho (Thr261) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51672 anti-SEK1 / MKK4 phospho (Thr261) antibody (left) or the same antibody preincubated with blocking peptide (right).
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ARG51672 anti-SEK1 / MKK4 phospho (Thr261) antibody ICC/IF image
Immunofluorescence: methanol-fixed HeLa cells stained with ARG51672 anti-SEK1 / MKK4 phospho (Thr261) antibody.
