ARG65579
anti-SDHB antibody
anti-SDHB antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes SDHB |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SDHB |
| Antigen Species | Human |
| Immunogen | Synthetic peptide of human SDHB |
| Conjugation | Un-conjugated |
| Alternate Names | Ip; SDH2; SDH1; IP; EC 1.3.5.1; PGL4; CWS2; Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial; SDHIP; Iron-sulfur subunit of complex II; SDH |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | 293T cell and mouse brain tissue, human fetal liver tissue and HepG2 cell, Jurkat cell |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by antigen-affinity chromatography. |
| Buffer | 1XPBS (pH 7.4), 0.05% Sodium azide and 40% Glycerol |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 40% Glycerol |
| Concentration | 1.3 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P21912 Human Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial Swiss-port # Q9CQA3 Mouse Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial |
|---|---|
| Gene Symbol | SDHB |
| Gene Full Name | succinate dehydrogenase complex, subunit B, iron sulfur (Ip) |
| Background | Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis. |
| Function | Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). [UniProt] |
| Highlight | Related products: SDHB antibodies; Anti-Rabbit IgG secondary antibodies; Related poster download: The Structure & Functions of Mitochondria.pdf |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 32 kDa |
Images (2) Click the Picture to Zoom In
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ARG65579 anti-SDHB antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human thyroid cancer tissue stained with ARG65579 anti-SDHB antibody (left) at 1:20 dilution, or the same antibody preincubated with synthetic peptide (right). (Original magnification: ×200)
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ARG65579 anti-SDHB antibody WB image
Western blot: 40 μg of 1) 293T, 2) Mouse brain tissue, 3) Human fetal liver, 4) HepG2 and 5) Jurkat lysates stained with ARG65579 anti-SDHB antibody at 1:300 dilution. Exposure time: 30 seconds.
