ARG65002
anti-SDHAF1 antibody
anti-SDHAF1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Metabolism antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes SDHAF1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Dog |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SDHAF1 |
| Antigen Species | Human |
| Immunogen | C-HDSTGAPETRPDGR |
| Conjugation | Un-conjugated |
| Alternate Names | Succinate dehydrogenase assembly factor 1, mitochondrial; SDHAF1; SDH assembly factor 1; LYRM8; LYR motif-containing protein 8 |
Application Instructions
| Application Suggestion |
|
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # A6NFY7 Human Succinate dehydrogenase assembly factor 1, mitochondrial |
|---|---|
| Background | The succinate dehydrogenase (SDH) complex (or complex II) of the mitochondrial respiratory chain is composed of 4 individual subunits. The protein encoded by this gene resides in the mitochondria, and is essential for SDH assembly, but does not physically associate with the complex in vivo. Mutations in this gene are associated with SDH-defective infantile leukoencephalopathy (mitochondrial complex II deficiency).[provided by RefSeq, Mar 2010] |
| Highlight | Related products: Anti-Goat IgG secondary antibodies; Related poster download: The Structure & Functions of Mitochondria.pdf |
| Research Area | Metabolism antibody |
| Calculated MW | 13 kDa |
Images (2) Click the Picture to Zoom In
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ARG65002 anti-SDHAF1 antibody WB image
Western Blot: Human Cerebellum lysate (35 µg protein in RIPA buffer) stained with ARG65002 anti-SDHAF1 antibody at 0.3 µg/ml dilution.
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ARG65002 anti-SDHAF1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human placenta tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG65002 anti-SDHAF1 antibody at 5 µg/ml dilution followed by AP-staining.
