ARG65526
anti-SCIMP antibody [NVL-07]
anti-SCIMP antibody [NVL-07] for Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Human
Immune System antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [NVL-07] recognizes SCIMP |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IP, WB |
| Specificity | The clone NVL-07 recognizes intracellular part of human transmembrane adaptor SCIMP. This protein of 17 kDa predicted Mw migrates as a 22 kDa band on SDS PAGE. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | NVL-07 |
| Isotype | IgG2a |
| Target Name | SCIMP |
| Antigen Species | Human |
| Immunogen | Recombinant intracellular part of human SCIMP |
| Conjugation | Un-conjugated |
| Alternate Names | SLP65/SLP76, Csk-interacting membrane protein; UNQ5783; C17orf87; SLP adapter and CSK-interacting membrane protein |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Intracellular staining. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from cell culture supernatant by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q6UWF3 Human SLP adapter and CSK-interacting membrane protein |
|---|---|
| Gene Symbol | SCIMP |
| Gene Full Name | SLP adaptor and CSK interacting membrane protein |
| Background | SCIMP (SLP adaptor and Csk interacting membrane protein), also known as Nvl, is a palmitoylated transmembrane adaptor protein expressed in professional antigen presenting cells, most prominently in the lymph nodes and spleen. It is associated with tetraspanin-enriched microdomains (together with MHC II). There is a close relationship between SCIMP and tyrosinkinase Lyn, which is constitutively bound to it by its SH3 domain. After MHC II-mediated stimulation in the immunological synapse SCIMP becomes phosphorylated at several tyrosine residues and provides docking sites for Grb2 and SLP65 or SLP76 adaptors transducing the signal downstream, as well as for the kinase Csk with modulatory roles. |
| Function | Lipid tetraspanin-associated transmembrane adapter/mediator involved in major histocompatibility complex (MHC) class II signaling transduction. Required in generating the calcium response and enhancing ERK activity upon MHC-II stimulation. [UniProt] |
| Research Area | Immune System antibody; Signaling Transduction antibody |
| Calculated MW | 17 kDa |
| PTM | Phosphorylated. Phosphorylation occurs on tyrosine residues by tyrosine-protein kinases LYN and SRC upon MHC-II stimulation. Palmitoylated. |
Images (1) Click the Picture to Zoom In
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ARG65526 anti-SCIMP antibody [NVL-07] FACS image
Flow Cytometry: A population of SCIMP transfected HEK293T cells stained with ARG65526 anti-SCIMP antibody [NVL-07]. The staining pattern reflects heterogeneity in the cell population regarding transfection efficiency.
