ARG43284
anti-SARS-CoV-2 Spike protein (S2) antibody
anti-SARS-CoV-2 Spike protein (S2) antibody for ICC/IF,Immunoprecipitation,Western blot and Virus
Overview
| Product Description | Rabbit Polyclonal antibody recognizes SARS-CoV-2 Spike protein (S2) |
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| Tested Reactivity | Virus |
| Tested Application | ICC/IF, IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SARS-CoV-2 Spike protein (S2) |
| Antigen Species | Virus |
| Immunogen | Recombinant fusion protein corresponding to aa. 686-1214 of SARS-CoV-2 Spike protein. |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Background | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronavirus viruses their crown-like appearance. This glycoprotein mediates attachment of the virus particle and entry into the host cell. S protein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests. |
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| Function | mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG43284 anti-SARS-CoV-2 Spike protein (S2) antibody ICC/IF image
Immunofluorescence: 293T cells transfected with SARS-CoV-2 Spike S2 fusion protein (left) and untransfected 293T cells (right). Cells were stained with ARG43284 anti-SARS-CoV-2 Spike protein (S2) antibody at 1:100 dilution.
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ARG43284 anti-SARS-CoV-2 Spike protein (S2) antibody WB image
Western blot: Untransfected 293T (right) and Spike S2 transfected 293T (left). 25 µg of cell lysates were stained with ARG43284 anti-SARS-CoV-2 Spike protein (S2) antibody at 1:5000 dilution.
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ARG43284 anti-SARS-CoV-2 Spike protein (S2) antibody IP image
Immunoprecipitation: 300 µg extracts of 293T cells were immunoprecipitated and stained with ARG43284 anti-SARS-CoV-2 Spike protein (S2) antibody at 1:5000 dilution.
