ARG43729
anti-SARS-CoV-2 ORF8 antibody
anti-SARS-CoV-2 ORF8 antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Virus
Overview
| Product Description | Rabbit Polyclonal antibody recognizesSARS-CoV-2 ORF8 |
|---|---|
| Tested Reactivity | Virus |
| Tested Application | ELISA, IHC-P, WB |
| Specificity | ORF8 Antibody is predicted to not cross-react with other coronavirus family members. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SARS-CoV-2 ORF8 |
| Antigen Species | Virus |
| Immunogen | Synthetic peptide corresponding to 14 amino acids near carboxyl terminus of SARS-CoV-2 (COVID-19) ORF8 protein. The immunogen is located within the last 50 amino acids of the SARS-CoV-2 (COVID-19) ORF8 protein. |
| Conjugation | Un-conjugated |
| Alternate Names | ORF8 protein, ns8, ORF8, Non-structural protein 8 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS and 0.02% Sodium azide. |
| Preservative | 0.02% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | ORF8 |
|---|---|
| Gene Full Name | SARS-CoV-2 ORF8 |
| Background | Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus. The disease is the cause of the 2019–20 coronavirus outbreak. SARS-CoV-2 virus proteins include structural proteins, non-structural proteins and accessory factors. The structure of SARS-CoV-2 consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. SARS-CoV-2 non-structural protein is ORF1ab that consists of 16 proteins (nsp1-nsp16), while accessory factors include ORF3a, ORF3b, ORF6, ORF7a, ORF7b, ORF8, ORF9b, ORF9c and ORF10. ORF8 may play a role in modulating host immune response (Probable). May play a role in blocking host IL17 cytokine by its interaction with host IL17RA. |
| Highlight | Related products: SARS-CoV antibodies; SARS-CoV ELISA Kits; SARS-CoV recombinant proteins; Anti-Rabbit IgG secondary antibodies; Related news: HMGB1, a biomarker and therapeutic target in COVID-19 |
Images (3) Click the Picture to Zoom In
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ARG43729 anti-SARS-CoV-2 ORF8 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded COVID-19 patient lung tissue (left) or health control (right) lung tissue were fixed with formaldehyde and blocked with 10% serum for 1 hour at RT. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). The tissue section was stained with ARG43729 anti-SARS-CoV-2 ORF8 at 0.1μg/mL dilution, overnight at 4°C. Counter stained with Hematoxylin.
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ARG43729 anti-SARS-CoV-2 ORF8 antibody WB image
Western blot: 30 ng of SARS-CoV-2 ORF8 recombinant protein stained with ARG43729 anti-SARS-CoV-2 ORF8 for 1 hour incubation at RT in 5% NFDM/TBST, at 1 µg/ml (left) or 2 µg/ml (right) dilution.
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ARG43729 anti-SARS-CoV-2 ORF8 antibody ELISA image
Direct ELISA: SARS-CoV-2 ORF8 rprotein was coated on the plate and ARG43729 anti-SARS-CoV-2 ORF8 antibody was used as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL.
