ARG64705

anti-SAR1 antibody

anti-SAR1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes SAR1
Tested Reactivity Hu, Ms
Predict Reactivity Rat
Tested Application IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name SAR1
Antigen Species Human
Immunogen C-HSRLVESKVELNA
Conjugation Un-conjugated
Alternate Names masra2; COPII-associated small GTPase; Sara; SARA1; SAR1; GTP-binding protein SAR1a

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P3 - 5 µg/ml
WB0.1 - 0.3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 56681 Human SAR1A

Swiss-port # Q9NR31 Human GTP-binding protein SAR1a

Gene Symbol SAR1A
Gene Full Name secretion associated, Ras related GTPase 1A
Function Involved in transport from the endoplasmic reticulum to the Golgi apparatus (By similarity). Required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining endoplasmic reticulum exit sites (ERES). [UniProt]
Research Area Signaling Transduction antibody
Calculated MW 22 kDa

Images (3) Click the Picture to Zoom In

  • ARG64705 anti-SAR1 antibody WB image

    Western Blot: NIH3T3 lysate (35 µg protein in RIPA buffer) stained with ARG64705 anti-SAR1 antibody at 0.1 µg/ml dilution.

  • ARG64705 anti-SAR1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human skin tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64705 anti-SAR1 antibody at 3.75 µg/ml dilution followed by AP-staining.

  • ARG64705 anti-SAR1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human small intestine tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64705 anti-SAR1 antibody at 3.75 µg/ml dilution followed by AP-staining.