ARG54072
anti-SAFB1 antibody
anti-SAFB1 antibody for ICC/IF,Immunoprecipitation,Western blot and Human
Gene Regulation antibody
Overview
| Product Description | Mouse Monoclonal antibody recognizes SAFB-1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Target Name | SAFB1 |
| Antigen Species | Human |
| Immunogen | Purified recombinant human SAFB1 protein fragments expressed in E.coli. |
| Conjugation | Un-conjugated |
| Alternate Names | HET; HSP27 estrogen response element-TATA box-binding protein; HSP27 ERE-TATA-binding protein; SAFB1; SAF-B; SAF-B1; Scaffold attachment factor B1; HAP |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 130 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl, 0.2% Sodium azide and 50% Glycerol |
| Preservative | 0.2% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SAFB |
| Gene Full Name | scaffold attachment factor B |
| Background | Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing By similarity. Can function as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. When associated with RBMX, binds to and stimulates transcription from the SREBF1 promoter By similarity. Can inhibit cell proliferation. |
| Function | Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing (By similarity). Can function as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. When associated with RBMX, binds to and stimulates transcription from the SREBF1 promoter (By similarity). Can inhibit cell proliferation. [UniProt] |
| Cellular Localization | Nucleus |
| Research Area | Gene Regulation antibody |
| Calculated MW | 103 kDa |
| PTM | Sumoylated by PIAS1 with SUMO1 and SUMO2/3, desumoylated by SENP1. Sumoylation is required for transcriptional repressor activity. |
Images (2) Click the Picture to Zoom In
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ARG54072 anti-SAFB1 antibody ICC/IF image
Immunofluorescence: HeLa cells fixed with 4% Paraformaldehyde and stained with ARG54072 anti-SAFB1 antibody at 1:200 dilution.
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ARG54072 anti-SAFB1 antibody WB image
Western blot: ZR75-1 cell lysate stained with ARG54072 anti-SAFB1 antibody at 1:4000 dilution.
