ARG51722
anti-S6 Ribosomal Protein phospho (Ser235) antibody
anti-S6 Ribosomal Protein phospho (Ser235) antibody for ICC/IF,Western blot and Human,Mouse,Rat
Gene Regulation antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes S6 Ribosomal Protein phospho (Ser235) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | S6 Ribosomal Protein |
| Antigen Species | Human |
| Immunogen | Peptide sequence around phosphorylation site of serine 235 (R-L-S(p)-S-L) derived from Human S6 Ribosomal Protein. |
| Conjugation | Un-conjugated |
| Alternate Names | Phosphoprotein NP33; 40S ribosomal protein S6; S6 |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Buffer | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | RPS6 |
| Gene Full Name | ribosomal protein S6 |
| Background | May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. |
| Function | May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. [UniProt] |
| Research Area | Gene Regulation antibody |
| Calculated MW | 29 kDa |
| PTM | Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex. |
Images (2) Click the Picture to Zoom In
-
ARG51722 anti-S6 Ribosomal Protein phospho (Ser235) antibody WB image
Western blot: Extracts from PC12 cells untreated or treated with anisomycin stained with ARG51722 anti-S6 Ribosomal Protein phospho (Ser235) antibody.
-
ARG51722 anti-S6 Ribosomal Protein phospho (Ser235) antibody ICC/IF image
Immunofluorescence: methanol-fixed HeLa cells stained with ARG51722 anti-S6 Ribosomal Protein phospho (Ser235) antibody.
