ARG52419
anti-S6 Ribosomal Protein phospho (Ser 244) antibody
anti-S6 Ribosomal Protein phospho (Ser 244) antibody for Western blot and Human,Mouse
Gene Regulation antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes S6 Ribosomal Protein phospho (Ser 244) |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Chk, Rat, Gpig, NHuPrm, Xenopus laevis, Zfsh |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | S6 Ribosomal Protein |
| Antigen Species | Mouse |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser244 conjugated to KLH |
| Conjugation | Un-conjugated |
| Alternate Names | Phosphoprotein NP33; 40S ribosomal protein S6; S6 |
Application Instructions
| Application Suggestion |
|
||||
|---|---|---|---|---|---|
| Application Note | Specific for the ~28k rpS6 protein phosphorylated at Ser244. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified |
| Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Stabilizer | 0.1 mg/ml BSA, 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | RPS6 |
| Gene Full Name | ribosomal protein S6 |
| Background | Ribosomal protein S6 (rpS6) is a critical component of the 40 S ribosomal subunit that mediates translation initiation at the 5′-m7 GpppG cap of mRNA. The rpS6 protein is both cytoplasmic and nuclear localized (Chen and Dittmer 2011). In response to mitogenic stimuli, rpS6 undergoes ordered C-terminal phosphorylation by p70 S6 kinases and p90 ribosomal S6 kinases on four Ser residues (Ser-235, Ser-236, Ser-240, and Ser-244) whose modification potentiates rpS6 cap binding activity (Hutchinson et al., 2011). Additionally, rpS6 phosphorylation and function are highly regulated and have been implicated in the regulation of translational initiation and protein synthesis in response to extracellular stimuli such as TRAIL and gamma interferon (IFN-γ), as well as upon activation of the phosphatidylinositol 3-kinase (PI3K)–Akt–mTOR pathway (Chen and Dittmer 2011). |
| Research Area | Gene Regulation antibody |
| Calculated MW | 29 kDa |
| PTM | Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex. |
Images (1) Click the Picture to Zoom In
-
ARG52419 anti-S6 Ribosomal Protein phospho (Ser 244) antibody WB image
Western blot: Jurkat cell lysate showing specific immunolabeling of the ~28k rpS6 phosphorylated at Ser 244 (Control) by using ARG52419 anti-S6 Ribosomal Protein phospho (Ser 244) antibody.
Phosphospecificity is shown in the second lane where immunolabeling is blocked by preadsorption of the phospho-peptide used as antigen (peptide).
