ARG51205
anti-S6 Ribosomal Protein antibody
anti-S6 Ribosomal Protein antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Gene Regulation antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes S6 Ribosomal Protein |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | S6 Ribosomal Protein |
| Antigen Species | Human |
| Immunogen | Peptide sequence around aa. 233~237 (R-L-S-S-L) derived from Human S6 Ribosomal Protein. |
| Conjugation | Un-conjugated |
| Alternate Names | Phosphoprotein NP33; 40S ribosomal protein S6; S6 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic peptide. Antibodies were purified by affinity-chromatography using epitope-specific peptide. |
| Buffer | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | RPS6 |
| Gene Full Name | ribosomal protein S6 |
| Background | May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. |
| Function | May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. [UniProt] |
| Research Area | Gene Regulation antibody |
| Calculated MW | 29 kDa |
| PTM | Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex. |
Images (3) Click the Picture to Zoom In
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ARG51205 anti-S6 Ribosomal Protein antibody WB image
Western blot: 20 µg of 293T and HeLa cell lysates stained with ARG51205 anti-S6 Ribosomal Protein antibody at 1:500 dilution.
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ARG51205 anti-S6 Ribosomal Protein antibody IHC-P image
Immunohistochemistry: paraffin- embedded human lung carcinoma tissue stained with anti-S6 Ribosomal Protein antibody ARG51205 (left) or the same antibody preincubated with blocking peptide (right).
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ARG51205 anti-S6 Ribosomal Protein antibody ICC/IF image
Immunofluorescence: methanol-fixed MCF7 cells stained with anti-S6 Ribosomal Protein antibody ARG51205.
Customer's Feedback
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Review for anti-S6 Ribosomal Protein antibody
Application：WB
Sample：293T and HeLa
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
