ARG63989
anti-S100A7 / Psoriasin antibody
anti-S100A7 / Psoriasin antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Controls and Markers antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes S100A7 / Psoriasin |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | S100A7 / Psoriasin |
| Antigen Species | Human |
| Immunogen | C-HKYTRRDDKIDKP |
| Conjugation | Un-conjugated |
| Alternate Names | S100A7c; S100 calcium-binding protein A7; PSOR1; Psoriasin; Protein S100-A7 |
Application Instructions
| Application Suggestion |
|
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein differs from the other S100 proteins of known structure in its lack of calcium binding ability in one EF-hand at the N-terminus. This protein is markedly over-expressed in the skin lesions of psoriatic patients, but is excluded as a candidate gene for familial psoriasis susceptibility. The exact function of this protein is not known. [provided by RefSeq, Jul 2008] |
| Research Area | Cancer antibody; Controls and Markers antibody; Signaling Transduction antibody |
| Calculated MW | 11 kDa |
Images (3) Click the Picture to Zoom In
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ARG63989 anti-S100A7 / Psoriasin antibody WB image
Western blot: Human Skin lysate (35 µg protein in RIPA buffer) stained with ARG63989 anti-S100A7 / Psoriasin antibody at 0.1 µg/ml dilution.
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ARG63989 anti-S100A7 / Psoriasin antibody WB image
Western blot: 10 µg of HEK293 cell lysate (in RIPA buffer) overexpressing Human S100A7 with C-terminal MYC tag stained with ARG63989 anti-S100A7 / Psoriasin antibody at 0.5 µg/ml dilution (Lane A); Mock-transfected HEK293 stained with primary antibodies (Lane B); Overexpressing HEK293 cell lysate stained with anti-MYC Tag at 1:1000 dilution (Lane C). Primary antibodies were incubated at RT for 1 hour.
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ARG63989 anti-S100A7 / Psoriasin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63989 anti-S100A7 / Psoriasin antibody at 3.75 µg/ml dilution followed by AP-staining.
