ARG41966
anti-S100A4 antibody [S100A4/1481]
anti-S100A4 antibody [S100A4/1481] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Overview
| Product Description | Mouse Monoclonal antibody [S100A4/1481] recognizes S100A4 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | S100A4/1481 |
| Isotype | IgG1, kappa |
| Target Name | S100A4 |
| Antigen Species | Human |
| Immunogen | Recombinant protein of Human S100A4. |
| Conjugation | Un-conjugated |
| Alternate Names | S100 calcium-binding protein A4; Placental calcium-binding protein; 42A; MTS1; FSP1; CAPL; Metastasin; Protein S100-A4; P9KA; 18A2; Protein Mts1; Calvasculin; PEL98 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | HeLa | ||||||||||
| Observed Size | ~ 12 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS, 0.05% Sodium azide and 0.1 mg/ml BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 0.1 mg/ml BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | S100A4 |
| Gene Full Name | S100 calcium binding protein A4 |
| Background | The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008] |
| Highlight | Related products: S100A4 antibodies; S100A4 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: New antibody panels for Myofibroblasts and CAFs |
| Calculated MW | 12 kDa |
Images (6) Click the Picture to Zoom In
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ARG41966 anti-S100A4 antibody [S100A4/1481] ICC/IF image
Immunofluorescence: Fixed and permeabilized HeLa cells stained with ARG41966 anti-S100A4 antibody [S100A4/1481] (green). Reddot (red) for nuclear staining.
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ARG41966 anti-S100A4 antibody [S100A4/1481] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human placenta tissue. Antigen Retrieval: Steam tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min followed by cooling. The tissue section was stained with ARG41966 anti-S100A4 antibody [S100A4/1481].
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ARG41966 anti-S100A4 antibody [S100A4/1481] WB image
Western blot: HeLa, A549 and A431 cell lysates stained with ARG41966 anti-S100A4 antibody [S100A4/1481].
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ARG41966 anti-S100A4 antibody [S100A4/1481] WB image
Western blot: HeLa cell lysate stained with ARG41966 anti-S100A4 antibody [S100A4/1481].
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ARG41966 anti-S100A4 antibody [S100A4/1481] FACS image
Flow Cytometry: Permeabilized T98G cells stained with ARG41966 anti-S100A4 antibody [S100A4/1481] (blue); Isotype control (red).
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ARG41966 anti-S100A4 antibody [S100A4/1481] FACS image
Flow Cytometry: Permeabilized A549 cells stained with ARG41966 anti-S100A4 antibody [S100A4/1481] (blue); Isotype control (red).
