ARG57728
anti-S100 beta antibody [4C4.9]
anti-S100 beta antibody [4C4.9] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Mouse Monoclonal antibody [4C4.9] recognizes S100 beta |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Specificity | This antibody is specific against an epitope located on the beta-chain (i.e. in S100A and S100B) but not on the alpha-chain of S-100 (i.e. in S100A and S100A0). |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 4C4.9 |
| Isotype | IgG2a, kappa |
| Target Name | S100 beta |
| Antigen Species | Bovine |
| Immunogen | Purified Bovine brain S100 protein. |
| Conjugation | Un-conjugated |
| Alternate Names | S-100 protein beta chain; NEF; S100; Protein S100-B; S100 calcium-binding protein B; S100beta; S-100 protein subunit beta; S100-B |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | ~ 11 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified. |
| Buffer | PBS, 0.05% Sodium azide and 0.1 mg/ml BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 0.1 mg/ml BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | S100B |
| Gene Full Name | S100 calcium binding protein B |
| Background | The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes. [provided by RefSeq, Jul 2008] |
| Function | Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity. [UniProt] |
| Calculated MW | 11 kDa |
Images (3) Click the Picture to Zoom In
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ARG57728 anti-S100 beta antibody [4C4.9] ICC/IF image
Immunofluorescence: A2058 cells stained with ARG57728 anti-S100 beta antibody [4C4.9] (green).
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ARG57728 anti-S100 beta antibody [4C4.9] IHC-P image
Immunohistochemistry: Paraffin-embedded Human melanoma stained with ARG57728 anti-S100 beta antibody [4C4.9].
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ARG57728 anti-S100 beta antibody [4C4.9] WB image
Western blot: Human brain lysate stained with ARG57728 anti-S100 beta antibody [4C4.9].
