ARG42666
anti-Ribonuclease Inhibitor antibody
anti-Ribonuclease Inhibitor antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Monkey
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Ribonuclease Inhibitor |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Mk |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Ribonuclease Inhibitor |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to aa. 2-37 of Human Ribonuclease Inhibitor. (SLDIQSLDIQCEELSDARWAELLPLLQQCQVVRLDD) |
| Conjugation | Un-conjugated |
| Alternate Names | RNH; Ribonuclease/angiogenin inhibitor 1; RAI; Ribonuclease inhibitor; Placental ribonuclease inhibitor; Placental RNase inhibitor |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 50 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | RNH1 |
| Gene Full Name | ribonuclease/angiogenin inhibitor 1 |
| Background | Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular RNases (summarized by Lee et al., 1988 [PubMed 3219362]). In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin (MIM 105850). Ribonuclease inhibitor, of 50,000 Da, binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo.[supplied by OMIM, Jul 2010] |
| Function | Ribonuclease inhibitor which inhibits RNASE1, RNASE2 and ANG. May play a role in redox homeostasis. [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
| Calculated MW | 50 kDa |
| PTM | The N-terminus is blocked. At least 30 of the 32 cysteine residues are in the reduced form. [UniProt] |
Images (7) Click the Picture to Zoom In
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ARG42666 anti-Ribonuclease Inhibitor antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human intestinal cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42666 anti-Ribonuclease Inhibitor antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG42666 anti-Ribonuclease Inhibitor antibody WB image
Western blot: 50 µg of samples under reducing conditions. Human placenta, HeLa, SK-OV-3, Jurkat, U-87 MG, COS-7, SW620 and Caco-2 whole cell lysates stained with ARG42666 anti-Ribonuclease Inhibitor antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG42666 anti-Ribonuclease Inhibitor antibody FACS image
Flow Cytometry: A549 cells were blocked with 10% normal goat serum and then stained with ARG42666 anti-Ribonuclease Inhibitor antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG42666 anti-Ribonuclease Inhibitor antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human intestinal cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42666 anti-Ribonuclease Inhibitor antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG42666 anti-Ribonuclease Inhibitor antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42666 anti-Ribonuclease Inhibitor antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG42666 anti-Ribonuclease Inhibitor antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42666 anti-Ribonuclease Inhibitor antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG42666 anti-Ribonuclease Inhibitor antibody FACS image
Flow Cytometry: A431 cells were blocked with 10% normal goat serum and then stained with ARG42666 anti-Ribonuclease Inhibitor antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
