ARG66073
anti-Resistin antibody
anti-Resistin antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Overview
| Product Description | Goat Polyclonal antibody recognizes Resistin |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ELISA, IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Resistin |
| Antigen Species | Human |
| Immunogen | E. coli derived recombinant Human Resistin. (SSKTLCSMEE AINERIQEVA GSLIFRAISS IGLECQSVTS RGDLATCPRG FAVTGCTCGS ACGSWDVRAE TTCHCQCAGM DWTGARCCRV QP) |
| Conjugation | Un-conjugated |
| Alternate Names | C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted protein A12-alpha-like 2; XCP1; Resistin; Adipose tissue-specific secretory factor; FIZZ3; RETN1; ADSF; Cysteine-rich secreted protein FIZZ3; RSTN |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.2) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | RETN |
| Gene Full Name | resistin |
| Background | This gene belongs to the family defined by the mouse resistin-like genes. The characteristic feature of this family is the C-terminal stretch of 10 cys residues with identical spacing. The mouse homolog of this protein is secreted by adipocytes, and may be the hormone potentially linking obesity to type II diabetes. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2010] |
| Function | Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes. [UniProt] |
| Calculated MW | 11 kDa |
Images (4) Click the Picture to Zoom In
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ARG66073 anti-Resistin antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human normal placenta. The recommended ARG66073 anti-Resistin antibody concentration is 1.0 µg/ml with a two hour incubation at RT. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
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ARG66073 anti-Resistin antibody WB image
Western blot: 250 - 0.24 ng of Human Resistin stained with ARG66073 anti-Resistin antibody, under non-reducing conditions.
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ARG66073 anti-Resistin antibody standard curve image
Sandwich ELISA: ARG66073 anti-Resistin antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG66074 anti-Resistin antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density reading at 405 - 650 nm.
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ARG66073 anti-Resistin antibody WB image
Western blot: 250 - 0.24 ng of Human Resistin stained with ARG66073 anti-Resistin antibody, under reducing conditions.
