ARG65989
anti-Resistin antibody [1.2_3D7-1D11]
anti-Resistin antibody [1.2_3D7-1D11] for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [1.2_3D7-1D11] recognizes Resistin |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1.2_3D7-1D11 |
| Isotype | IgG2b, kappa |
| Target Name | Resistin |
| Antigen Species | Human |
| Immunogen | E. coli derived recombinant Human Resistin. (SSKTLCSMEE AINERIQEVA GSLIFRAISS IGLECQSVTS RGDLATCPRG FAVTGCTCGS ACGSWDVRAE TTCHCQCAGM DWTGARCCRV QP) |
| Conjugation | Un-conjugated |
| Alternate Names | C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted protein A12-alpha-like 2; XCP1; Resistin; Adipose tissue-specific secretory factor; FIZZ3; RETN1; ADSF; Cysteine-rich secreted protein FIZZ3; RSTN |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.2) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | RETN |
| Gene Full Name | resistin |
| Background | This gene belongs to the family defined by the mouse resistin-like genes. The characteristic feature of this family is the C-terminal stretch of 10 cys residues with identical spacing. The mouse homolog of this protein is secreted by adipocytes, and may be the hormone potentially linking obesity to type II diabetes. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2010] |
| Function | Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes. [UniProt] |
| Highlight | Related products: Resistin antibodies; Resistin ELISA Kits; Anti-Mouse IgG secondary antibodies; Related news: A new role of fat – factory of circulating miRNAs |
| Calculated MW | 11 kDa |
Images (4) Click the Picture to Zoom In
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ARG65989 anti-Resistin antibody [1.2_3D7-1D11] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human normal placenta. The recommended ARG65989 anti-Resistin antibody [1.2_3D7-1D11] concentration is 1.0 µg/ml with a two hour incubation at RT. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
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ARG65989 anti-Resistin antibody [1.2_3D7-1D11] WB image
Western blot: 250 - 0.24 ng (left to right) of Human Resistin stained with ARG65989 anti-Resistin antibody [1.2_3D7-1D11], under non-reducing conditions.
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ARG65989 anti-Resistin antibody [1.2_3D7-1D11] standard curve image
Sandwich ELISA: ARG65989 anti-Resistin antibody [1.2_3D7-1D11] as a capture antibody at 4.0 - 8.0 µg/ml combined with ARG66074 anti-Resistin antibody (Biotin) as a detection antibody at 0.5 - 1.0 µg/ml. Results of a typical standard run with optical density reading at 405 - 650 nm.
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ARG65989 anti-Resistin antibody [1.2_3D7-1D11] WB image
Western blot: 250 - 0.24 ng (left to right) of Human Resistin stained with ARG65989 anti-Resistin antibody [1.2_3D7-1D11], under reducing conditions.
