ARG67136
anti-Rb1 / Retinoblastoma protein antibody
anti-Rb1 / Retinoblastoma protein antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Gene Regulation antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Rb1 / Retinoblastoma protein |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Rb1 / Retinoblastoma protein |
| Antigen Species | Human |
| Immunogen | Synthetic peptide of Human Rb1 / Retinoblastoma protein. |
| Conjugation | Un-conjugated |
| Alternate Names | RB1; RB Transcriptional Corepressor 1; PPP1R130; RB; Protein Phosphatase 1, Regulatory Subunit 130; Prepro-Retinoblastoma-Associated Protein; Retinoblastoma-Associated Protein; Retinoblastoma 1; P110-RB1; P105-Rb; Pp110; OSRC; PRb; Retinoblastoma Suspectibility Protein; Osteosarcoma; Rb |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity chromatography purified |
| Buffer | PBS, 0.02% Sodium azide, 0.5% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA, 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | RB1 |
| Gene Full Name | RB Transcriptional Corepressor 1 |
| Background | The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008] |
| Function | Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. (Microbial infection) In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Gene Regulation antibody |
| Calculated MW | 106 kDa |
| PTM | Acetylation, Methylation, Phosphoprotein. [UniProt] |
Images (5) Click the Picture to Zoom In
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ARG67136 anti-Rb1 / Retinoblastoma protein antibody IHC-P image
Immunohistochemistry: Rat liver stained with ARG67136 anti-Rb1 / Retinoblastoma protein antibody at 1:200 dilution.
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ARG67136 anti-Rb1 / Retinoblastoma protein antibody IHC-P image
Immunohistochemistry: Human colon stained with ARG67136 anti-Rb1 / Retinoblastoma protein antibody at 1:200 dilution.
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ARG67136 anti-Rb1 / Retinoblastoma protein antibody ICC/IF image
Immunofluorescence: HeLa stained with ARG67136 anti-Rb1 / Retinoblastoma protein antibody at 1:200 dilution.
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ARG67136 anti-Rb1 / Retinoblastoma protein antibody WB image
Western blot: HepG2 stained with ARG67136 anti-Rb1 / Retinoblastoma protein antibody at 1:1000 dilution.
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ARG67136 anti-Rb1 / Retinoblastoma protein antibody IHC-P image
Immunohistochemistry: Mouse liver stained with ARG67136 anti-Rb1 / Retinoblastoma protein antibody at 1:200 dilution.
