ARG58296
anti-RUNX1T1 / ETO antibody
anti-RUNX1T1 / ETO antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes RUNX1T1 / ETO |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RUNX1T1 / ETO |
| Antigen Species | Human |
| Immunogen | E. coli-derived Human RUNX1T1/ETO recombinant protein (Position: T335-D510). |
| Conjugation | Un-conjugated |
| Alternate Names | AML1T1; MTG8; CBFA2T1; ZMYND2; Protein MTG8; Protein CBFA2T1; Cyclin-D-related protein; Eight twenty one protein; CDR; AML1-MTG8; Protein ETO; Zinc finger MYND domain-containing protein 2; ETO |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | RUNX1T1 |
| Gene Full Name | runt-related transcription factor 1; translocated to, 1 (cyclin D-related) |
| Background | This gene encodes a member of the myeloid translocation gene family which interact with DNA-bound transcription factors and recruit a range of corepressors to facilitate transcriptional repression. The t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities in acute myeloid leukemia. The translocation produces a chimeric gene made up of the 5'-region of the runt-related transcription factor 1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010] |
| Function | Transcription regulator that excerts its function by binding to histone deacetylases and transcription factors. Can repress transactivation mediated by TCF12. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 68 kDa |
Images (5) Click the Picture to Zoom In
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ARG58296 anti-RUNX1T1 / ETO antibody ICC/IF image
Immunofluorescence: U2OS cells were blocked with 10% goat serum and then stained with ARG58296 anti-RUNX1T1 / ETO antibody (green) at 2 µg/ml dilution, overnight at 4°C.
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ARG58296 anti-RUNX1T1 / ETO antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human mammary cancer. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58296 anti-RUNX1T1 / ETO antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG58296 anti-RUNX1T1 / ETO antibody WB image
Western blot: 50 µg of Human placenta and Mouse testis lysates stained with ARG58296 anti-RUNX1T1 / ETO antibody at 0.5 µg/ml, overnight at 4°C, under reducing conditions.
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ARG58296 anti-RUNX1T1 / ETO antibody FACS image
Flow Cytometry: U2OS cells were blocked with 10% normal goat serum and then stained with ARG58296 anti-RUNX1T1 / ETO antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG58296 anti-RUNX1T1 / ETO antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse brain tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58296 anti-RUNX1T1 / ETO antibody at 1 µg/ml dilution, overnight at 4°C.
