ARG59086
anti-RTKN / Rhotekin antibody
anti-RTKN / Rhotekin antibody for ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes RTKN / Rhotekin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RTKN / Rhotekin |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 254-513 of Human RTKN / Rhotekin (NP_001015056.1). |
| Conjugation | Un-conjugated |
| Alternate Names | Rhotekin |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | A431 | ||||||
| Observed Size | 65kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | RTKN |
| Gene Full Name | rhotekin |
| Background | This gene encodes a scaffold protein that interacts with GTP-bound Rho proteins. Binding of this protein inhibits the GTPase activity of Rho proteins. This protein may interfere with the conversion of active, GTP-bound Rho to the inactive GDP-bound form by RhoGAP. Rho proteins regulate many important cellular processes, including cytokinesis, transcription, smooth muscle contraction, cell growth and transformation. Dysregulation of the Rho signal transduction pathway has been implicated in many forms of cancer. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| Function | Mediates Rho signaling to activate NF-kappa-B and may confer increased resistance to apoptosis to cells in gastric tumorigenesis. May play a novel role in the organization of septin structures. [UniProt] |
| Calculated MW | 63 kDa |
Images (2) Click the Picture to Zoom In
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ARG59086 anti-RTKN / Rhotekin antibody ICC/IF image
Immunofluorescence: MCF7 cells stained with ARG59086 anti-RTKN / Rhotekin antibody.
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ARG59086 anti-RTKN / Rhotekin antibody WB image
Western blot: 25 µg of A431 cell lysate stained with ARG59086 anti-RTKN / Rhotekin antibody at 1:1000 dilution.
