ARG66670
anti-RPS4Y1 antibody
anti-RPS4Y1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes RPS4Y1 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RPS4Y1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide within aa. 190-270 of Human RPS4Y1. |
| Conjugation | Un-conjugated |
| Alternate Names | RPS4Y; 40S ribosomal protein S4, Y isoform 1; S4 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: High-pressure and temperature Tris-EDTA buffer (pH 8.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | HUVEC | ||||||||
| Observed Size | ~ 30 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol and 0.5% BSA |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P22090 Human 40S ribosomal protein S4, Y isoform 1 |
|---|---|
| Gene Symbol | RPS4Y1 |
| Gene Full Name | ribosomal protein S4, Y-linked 1 |
| Background | Cytoplasmic ribosomes, organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes ribosomal protein S4, a component of the 40S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene and ribosomal protein S4, X-linked (RPS4X). The 2 isoforms encoded by these genes are not identical, but are functionally equivalent. Ribosomal protein S4 belongs to the S4E family of ribosomal proteins. It has been suggested that haploinsufficiency of the ribosomal protein S4 genes plays a role in Turner syndrome; however, this hypothesis is controversial. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008] |
| Calculated MW | 29 kDa |
Images (4) Click the Picture to Zoom In
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ARG66670 anti-RPS4Y1 antibody ICC/IF image
Immunofluorescence: HUVEC cells stained with ARG66670 anti-RPS4Y1 antibody. The picture on the right was blocked with the synthetic peptide.
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ARG66670 anti-RPS4Y1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human brain tissue. Antigen Retrieval: High-pressure and temperature Tris-EDTA buffer (pH 8.0). Tissue section was stained with ARG66670 anti-RPS4Y1 antibody at 1:100 dilution, overnight at 4°C. The picture on the right was blocked with the synthetic peptide.
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ARG66670 anti-RPS4Y1 antibody WB image
Western blot: HUVEC cell lysate stained with ARG66670 anti-RPS4Y1 antibody at 1:1000 dilution.
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ARG66670 anti-RPS4Y1 antibody WB image
Western blot: 293T and Mouse spleen lysates stained with ARG66670 anti-RPS4Y1 antibody at 1:1000 dilution.
