ARG63838
anti-RPS19 antibody
anti-RPS19 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Gene Regulation antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes RPS19 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RPS19 |
| Antigen Species | Human |
| Immunogen | C-KMVEKDQDGGRK |
| Conjugation | Un-conjugated |
| Alternate Names | DBA; 40S ribosomal protein S19; S19; DBA1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S19E family of ribosomal proteins. It is located in the cytoplasm. Mutations in this gene cause Diamond-Blackfan anemia (DBA), a constitutional erythroblastopenia characterized by absent or decreased erythroid precursors, in a subset of patients. This suggests a possible extra-ribosomal function for this gene in erythropoietic differentiation and proliferation, in addition to its ribosomal function. Higher expression levels of this gene in some primary colon carcinomas compared to matched normal colon tissues has been observed. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008] |
| Research Area | Gene Regulation antibody |
| Calculated MW | 16 kDa |
Images (4) Click the Picture to Zoom In
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ARG63838 anti-RPS19 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human cortex tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63838 anti-RPS19 antibody at 5 µg/ml dilution followed by AP-staining.
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ARG63838 anti-RPS19 antibody WB image
Western blot: 35 µg of K562 cell lysate (in RIPA buffer) stained with ARG63838 anti-RPS19 antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
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ARG63838 anti-RPS19 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63838 anti-RPS19 antibody at 5 µg/ml dilution followed by AP-staining.
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ARG63838 anti-RPS19 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63838 anti-RPS19 antibody at 5 µg/ml dilution followed by AP-staining.
