ARG43884

anti-RPS13 antibody

anti-RPS13 antibody for ELISA,Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes RPS13
Tested Reactivity Hu
Tested Application ELISA, FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name RPS13
Antigen Species Human
Immunogen Human RPS13 recombinant protein
Expression System E.coli
Conjugation Un-conjugated
Protein Full Name Small ribosomal subunit protein uS15
Alternate Names RPS13; Ribosomal Protein S13; 40S Ribosomal Protein S13; S13; Small Ribosomal Subunit Protein US15; US15

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA0.1-0.5 µg/ml
FACS1-3 µg/1x10^6
IHC-P2-5 µg/ml
WB0.25-0.5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified with Immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose.
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 6207 Human RPS13

Swiss-port # P62277 Human 40S ribosomal protein S13

Gene Symbol RPS13
Gene Full Name Ribosomal Protein S13
Background Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S15P family of ribosomal proteins. It is located in the cytoplasm. The protein has been shown to bind to the 5.8S rRNA in rat. The gene product of the E. coli ortholog (ribosomal protein S15) functions at early steps in ribosome assembly. This gene is co-transcribed with two U14 small nucleolar RNA genes, which are located in its third and fifth introns. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Function Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.
Cellular Localization Cytoplasm, Nucleus
Calculated MW 17 kDa
PTM Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Images (4) Click the Picture to Zoom In

  • ARG43884 anti-RPS13 antibody IHC-P image

    mmunohistochemistry: Human colon adenocarcinoma stained with ARG43884 anti-RPS13 antibody at 2 μg/ml dilution.

  • ARG43884 anti-RPS13 antibody WB image

    Western blot: HepG2 and MCF-7 stained with ARG43884 anti-RPS13 antibody at 0.5 μg/mL dilution.

  • ARG43884 anti-RPS13 antibody FACS image

    Flow Cytometry: HL-60 cells stained withARG43884 anti-RPS13 antibody (blue) at 1 μg/1x10^6 cells dilution.

  • ARG43884 anti-RPS13 antibody WB image

    Western blot: HeLa cells stained with ARG43884 anti-RPS13 antibody at 0.5 μg/mL dilution.