ARG59794

anti-RPL14 antibody

anti-RPL14 antibody for ICC/IF,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes RPL14
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name RPL14
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 1-215 of Human RPL14 (NP_003964.3).
Conjugation Un-conjugated
Alternate Names 60S ribosomal protein L14; L14; RL14; CTG-B33; hRL14; CAG-ISL 7; CAG-ISL-7

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse ovary and 293T
Observed Size 23 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 67115 Mouse RPL14

GeneID: 9045 Human RPL14

Swiss-port # P50914 Human 60S ribosomal protein L14

Swiss-port # Q9CR57 Mouse 60S ribosomal protein L14

Gene Symbol RPL14
Gene Full Name ribosomal protein L14
Background Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L14E family of ribosomal proteins. It contains a basic region-leucine zipper (bZIP)-like domain. The protein is located in the cytoplasm. This gene contains a trinucleotide (GCT) repeat tract whose length is highly polymorphic; these triplet repeats result in a stretch of alanine residues in the encoded protein. Transcript variants utilizing alternative polyA signals and alternative 5'-terminal exons exist but all encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
Calculated MW 23 kDa

Images (2) Click the Picture to Zoom In

  • ARG59794 anti-RPL14 antibody WB image

    Western blot: 25 µg of Mouse ovary and 293T cell lysates stained with ARG59794 anti-RPL14 antibody at 1:1000 dilution.

  • ARG59794 anti-RPL14 antibody WB image

    Western blot: 25 µg of 293T cell lysate stained with ARG59794 anti-RPL14 antibody at 1:1000 dilution through one-step method.