ARG41578
anti-RPA2 / RPA32 antibody
anti-RPA2 / RPA32 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes RPA2 / RPA32 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RPA2 / RPA32 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 80-270 of Human RPA2 / RPA32 (NP_002937.1). |
| Conjugation | Un-conjugated |
| Alternate Names | RF-A protein 2; RPA32; RP-A p34; Replication protein A 34 kDa subunit; Replication factor A protein 2; Replication protein A 32 kDa subunit; REPA2; RP-A p32; RFA2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Positive Control | C6 | ||||||||||
| Observed Size | ~ 34 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P15927 Human Replication protein A 32 kDa subunit Swiss-port # Q63528 Rat Replication protein A 32 kDa subunit |
|---|---|
| Gene Symbol | RPA2 |
| Gene Full Name | replication protein A2, 32kDa |
| Function | As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. [UniProt] |
| Cellular Localization | Nucleus. Nucleus, PML body. Note=Redistributes to discrete nuclear foci upon DNA damage in an ATR-dependent manner. [UniProt] |
| Calculated MW | 29 kDa |
| PTM | Differentially phosphorylated throughout the cell cycle, becoming phosphorylated at the G1-S transition and dephosphorylated in late mitosis. Mainly phosphorylated at Ser-23 and Ser-29, by cyclin A-CDK2 and cyclin B-CDK1, respectively during DNA replication and mitosis. Dephosphorylation may require the serine/threonine-protein phosphatase 4. Phosphorylation at Ser-23 and Ser-29 is a prerequisite for further phosphorylation. Becomes hyperphosphorylated on additional residues including Ser-4, Ser-8, Thr-21 and Ser-33 in response to DNA damage. Hyperphosphorylation is mediated by ATM, ATR and PRKDC. Primarily recruited to DNA repair nuclear foci as a hypophosphorylated form it undergoes subsequent hyperphosphorylation, catalyzed by ATR. Hyperphosphorylation is required for RAD51 recruitment to chromatin and efficient DNA repair. Phosphorylation at Thr-21 depends upon RFWD3 presence. DNA damage-induced 'Lys-63'-linked polyubiquitination by PRPF19 mediates ATRIP recruitment to the RPA complex at sites of DNA damage and activation of ATR. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG41578 anti-RPA2 / RPA32 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human kidney cancer tissue stained with ARG41578 anti-RPA2 / RPA32 antibody at 1:100 dilution.
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ARG41578 anti-RPA2 / RPA32 antibody WB image
Western blot: 25 µg of C6 cell lysate stained with ARG41578 anti-RPA2 / RPA32 antibody at 1:1000 dilution.
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ARG41578 anti-RPA2 / RPA32 antibody IP image
Immunoprecipitation: 200 µg extracts of Jurkat cells were immunoprecipitated and stained with ARG41578 anti-RPA2 / RPA32 antibody at 1:1000 dilution.
