ARG59435
anti-RNF43 antibody
anti-RNF43 antibody for Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes RNF43 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RNF43 |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to G24-Y197 of Human RNF43. |
| Conjugation | Un-conjugated |
| Alternate Names | RING finger protein 43; URCC; E3 ubiquitin-protein ligase RNF43; EC 6.3.2.-; RNF124 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | RNF43 |
| Gene Full Name | ring finger protein 43 |
| Background | The protein encoded by this gene is a RING-type E3 ubiquitin ligase and is predicted to contain a transmembrane domain, a protease-associated domain, an ectodomain, and a cytoplasmic RING domain. This protein is thought to negatively regulate Wnt signaling, and expression of this gene results in an increase in ubiquitination of frizzled receptors, an alteration in their subcellular distribution, resulting in reduced surface levels of these receptors. Mutations in this gene have been reported in multiple tumor cells, including colorectal and endometrial cancers. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015] |
| Function | E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination, endocytosis and subsequent degradation of Wnt receptor complex components Frizzled. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone. [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Nucleus envelope. Note=According to a report, may be secreted. [UniProt] |
| Calculated MW | 86 kDa |
| PTM | Autoubiquitinated. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG59435 anti-RNF43 antibody WB image
Western blot: 50 µg of samples under reducing conditions. HeLa, 293T, COLO-320, SW620 and MCF-7 whole cell lysates stained with ARG59435 anti-RNF43 antibody at 0.5 µg/ml, overnight at 4°C.
