ARG63286
anti-RNF36 / TRIM69 antibody
anti-RNF36 / TRIM69 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cell Biology and Cellular Response antibody; Cell Death antibody; Developmental Biology antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes RNF36 / TRIM69 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Cow, Rat, Dog, Pig |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_892030.3; NP_542783.2). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RNF36 / TRIM69 |
| Antigen Species | Human |
| Immunogen | C-GENKEPLHILHPQ |
| Conjugation | Un-conjugated |
| Alternate Names | RFP-like domain-containing protein trimless; HSD34; EC 6.3.2.-; E3 ubiquitin-protein ligase TRIM69; Trif; Tripartite motif-containing protein 69; HSD-34; RNF36; RING finger protein 36 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q80X56 Mouse E3 ubiquitin-protein ligase TRIM69 Swiss-port # Q86WT6 Human E3 ubiquitin-protein ligase TRIM69 |
|---|---|
| Background | This gene encodes a member of the RING-B-box-coiled-coil (RBCC) family and encodes a protein with an N-terminal RING finger motif, a PRY domain and a C-terminal SPRY domain. The mouse ortholog of this gene is specifically expressed in germ cells at the round spermatid stages during spermatogenesis and, when overexpressed, induces apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008] |
| Research Area | Cell Biology and Cellular Response antibody; Cell Death antibody; Developmental Biology antibody |
| Calculated MW | 57 kDa |
| PTM | Phosphorylated. Phosphorylation is necessary for nuclear localization. |
Images (3) Click the Picture to Zoom In
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ARG63286 anti-RNF36 / TRIM69 antibody WB image
Western Blot: Mouse Testis lysate (35 µg protein in RIPA buffer) stained with ARG63286 anti-RNF36 / TRIM69 antibody at 0.5 µg/ml dilution.
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ARG63286 anti-RNF36 / TRIM69 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human cerebellum tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63286 anti-RNF36 / TRIM69 antibody at 2.5 µg/ml dilution followed by AP-staining.
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ARG63286 anti-RNF36 / TRIM69 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Testis. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63286 anti-RNF36 / TRIM69 antibody at 3.8 µg/ml dilution followed by AP-staining.
