ARG63286

anti-RNF36 / TRIM69 antibody

anti-RNF36 / TRIM69 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Cell Biology and Cellular Response antibody; Cell Death antibody; Developmental Biology antibody

Overview

Product Description Goat Polyclonal antibody recognizes RNF36 / TRIM69
Tested Reactivity Hu, Ms
Predict Reactivity Cow, Rat, Dog, Pig
Tested Application IHC-P, WB
Specificity This antibody is expected to recognize both reported isoforms (NP_892030.3; NP_542783.2).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name RNF36 / TRIM69
Antigen Species Human
Immunogen C-GENKEPLHILHPQ
Conjugation Un-conjugated
Alternate Names RFP-like domain-containing protein trimless; HSD34; EC 6.3.2.-; E3 ubiquitin-protein ligase TRIM69; Trif; Tripartite motif-containing protein 69; HSD-34; RNF36; RING finger protein 36

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P2 - 4 µg/ml
WB0.5 - 2 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 140691 Human TRIM69

GeneID: 70928 Mouse TRIM69

Swiss-port # Q80X56 Mouse E3 ubiquitin-protein ligase TRIM69

Swiss-port # Q86WT6 Human E3 ubiquitin-protein ligase TRIM69

Background This gene encodes a member of the RING-B-box-coiled-coil (RBCC) family and encodes a protein with an N-terminal RING finger motif, a PRY domain and a C-terminal SPRY domain. The mouse ortholog of this gene is specifically expressed in germ cells at the round spermatid stages during spermatogenesis and, when overexpressed, induces apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
Research Area Cell Biology and Cellular Response antibody; Cell Death antibody; Developmental Biology antibody
Calculated MW 57 kDa
PTM Phosphorylated. Phosphorylation is necessary for nuclear localization.

Images (3) Click the Picture to Zoom In

  • ARG63286 anti-RNF36 / TRIM69 antibody WB image

    Western Blot: Mouse Testis lysate (35 µg protein in RIPA buffer) stained with ARG63286 anti-RNF36 / TRIM69 antibody at 0.5 µg/ml dilution.

  • ARG63286 anti-RNF36 / TRIM69 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human cerebellum tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63286 anti-RNF36 / TRIM69 antibody at 2.5 µg/ml dilution followed by AP-staining.

  • ARG63286 anti-RNF36 / TRIM69 antibody IHC-P image

    Immunohistochemistry: paraffin embedded Human Testis. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63286 anti-RNF36 / TRIM69 antibody at 3.8 µg/ml dilution followed by AP-staining.