ARG42415
anti-RLTPR antibody [EM-53] (PE)
anti-RLTPR antibody [EM-53] (PE) for Flow cytometry and Human,Mouse
Overview
| Product Description | PE-conjugated Mouse Monoclonal antibody [EM-53] recognizes RLTPR |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | FACS |
| Specificity | The mouse monoclonal antibody EM-53 recognizes RLTPR / CARMIL2, an intracellular protein playing a role in actin filament elongation. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | EM-53 |
| Isotype | IgG1, kappa |
| Target Name | RLTPR |
| Antigen Species | Mouse |
| Immunogen | Murine RLTPR. |
| Conjugation | PE |
| Alternate Names | CARMIL2b; CARMIL2; Leucine-rich repeat-containing protein 16C; LRRC16C; RGD, leucine-rich repeat, tropomodulin and proline-rich-containing protein |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified |
| Buffer | PBS and 15 mM Sodium azide. |
| Preservative | 15 mM Sodium azide |
| Concentration | 0.1 mg/ml |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q3V3V9 Mouse Leucine-rich repeat-containing protein 16C Swiss-port # Q6F5E8 Human Leucine-rich repeat-containing protein 16C |
|---|---|
| Gene Symbol | RLTPR |
| Gene Full Name | RGD motif, leucine rich repeats, tropomodulin domain and proline-rich containing |
| Background | This gene encodes a member of the CARMIL (capping protein, Arp2/3, myosin-I linker) family of proteins. The encoded protein interacts with and negatively regulates the heterodimeric capping protein and promotes cell migration. Reduced expression of this gene has been observed in human psoriasis patients. Mutations in this gene cause a human immunodeficiency syndrome characterized by smooth muscle tumors and impaired T-cell function. [provided by RefSeq, May 2017] |
| Function | Cell membrane-cytoskeleton-associated protein that plays a role in the regulation of actin polymerization at the barbed end of actin filaments. Prevents F-actin heterodimeric capping protein (CP) activity at the leading edges of migrating cells, and hence generates uncapped barbed ends and enhances actin polymerization (PubMed:26466680). Plays a role in cell protrusion formations; involved in cell polarity, lamellipodial assembly, membrane ruffling and macropinosome formations (PubMed:19846667, PubMed:26578515, PubMed:26466680). Involved as well in cell migration and invadopodia formation during wound healing (PubMed:19846667, PubMed:26578515, PubMed:26466680). Required for CD28-mediated stimulation of NF-kappa-B signaling, involved in naive T cells activation, maturation into T memory cells, and differentiation into T helper and T regulatory cells (PubMed:27647349, PubMed:27647348, PubMed:28112205). [UniProt] |
| Cellular Localization | Isoform 2: Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium. Cell projection, ruffle. Note=Colocalizes to dynamic vimentin filaments both in the central cytoplasm and at leading edges of migrating cells (PubMed:26578515, PubMed:26466680, PubMed:19846667). Colocalizes with F-actin, Arp2/3 complex and cortactin to leading edge lamellipodia, ruffles and macropinosomes of migrating cells (PubMed:26578515). [UniProt] |
| Calculated MW | 155 kDa |
Images (1) Click the Picture to Zoom In
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ARG42415 anti-RLTPR antibody [EM-53] (PE) FACS image
Flow Cytometry: Separation of stained RLTPR transfected cells (red) from unstained RLTPR transfected cells (black). Cells were stained with ARG42415 anti-RLTPR antibody [EM-53] (PE) at 27 µg/ml dilution.
