ARG64977

anti-RIP140 antibody

anti-RIP140 antibody for Western blot and Human

Gene Regulation antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes RIP140
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Cow, Dog
Tested Application WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name RIP140
Antigen Species Human
Immunogen C-EKDSPRLTKTNP
Conjugation Un-conjugated
Alternate Names Nuclear factor RIP140; RIP140; Nuclear receptor-interacting protein 1; Receptor-interacting protein 140

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.5 - 2 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8204 Human NRIP1

Swiss-port # P48552 Human Nuclear receptor-interacting protein 1

Background Nuclear receptor interacting protein 1 (NRIP1) is a nuclear protein that specifically interacts with the hormone-dependent activation domain AF2 of nuclear receptors. Also known as RIP140, this protein modulates transcriptional activity of the estrogen receptor. [provided by RefSeq, Jul 2008]
Research Area Gene Regulation antibody; Signaling Transduction antibody
Calculated MW 127 kDa
PTM Acetylation regulates its nuclear translocation and corepressive activity (By similarity). Acetylation abolishes interaction with CTBP1. Phosphorylation enhances interaction with YWHAH.

Images (1) Click the Picture to Zoom In

  • ARG64977 anti-RIP140 antibody WB image

    Western Blot: Human Cerebellum lysate (35 µg protein in RIPA buffer) stained with ARG64977 anti-RIP140 antibody at 0.5 µg/ml dilution.