ARG63778
anti-RICTOR antibody
anti-RICTOR antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
Cell Biology and Cellular Response antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes RICTOR |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Dog |
| Tested Application | IHC-P |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RICTOR |
| Antigen Species | Human |
| Immunogen | C-KQPIVDTSAES |
| Conjugation | Un-conjugated |
| Alternate Names | AVO3 homolog; PIA; Rapamycin-insensitive companion of mTOR; hAVO3; AVO3 |
Application Instructions
| Application Suggestion |
|
||||
|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q6R327 Human Rapamycin-insensitive companion of mTOR |
|---|---|
| Background | RICTOR and MTOR (FRAP1; MIM 601231) are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth (Sarbassov et al., 2004 [PubMed 15268862]).[supplied by OMIM, Mar 2008] |
| Research Area | Cell Biology and Cellular Response antibody; Signaling Transduction antibody |
| Calculated MW | 192 kDa |
| PTM | Phosphorylated by MTOR; when part of mTORC2. Phosphorylated at Thr-1135 by RPS6KB1; phosphorylation of RICTOR inhibits mTORC2 and AKT1 signaling. |
Images (1) Click the Picture to Zoom In
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ARG63778 anti-RICTOR antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Kidney. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63778 anti-RICTOR antibody at 2.5 µg/ml dilution followed by AP-staining.
