ARG58777
anti-RFX5 antibody
anti-RFX5 antibody for Western blot and Human,Mouse
Overview
| Product Description | Goat Polyclonal antibody recognizes RFX5 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Cow, Rat, Dog, Pig |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RFX5 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide from the internal region of Human RFX5 (NP_000440.1). (C-TGDKSSEPSTLSNE) |
| Conjugation | Un-conjugated |
| Alternate Names | DNA-binding protein RFX5; Regulatory factor X 5 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 73 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | RFX5 |
| Gene Full Name | regulatory factor X, 5 (influences HLA class II expression) |
| Background | A lack of MHC-II expression results in a severe immunodeficiency syndrome called MHC-II deficiency, or the bare lymphocyte syndrome (BLS; MIM 209920). At least 4 complementation groups have been identified in B-cell lines established from patients with BLS. The molecular defects in complementation groups B, C, and D all lead to a deficiency in RFX, a nuclear protein complex that binds to the X box of MHC-II promoters. The lack of RFX binding activity in complementation group C results from mutations in the RFX5 gene encoding the 75-kD subunit of RFX (Steimle et al., 1995). RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been found but the full-length natures of only two have been determined. [provided by RefSeq, Jul 2008] |
| Function | Activates transcription from class II MHC promoters. Recognizes X-boxes. Mediates cooperative binding between RFX and NF-Y. RFX binds the X1 box of MHC-II promoters. [UniProt] |
| Calculated MW | 65 kDa |
| PTM | Phosphorylated. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG58777 anti-RFX5 antibody WB image
Western blot: 35 µg of Daudi cell lysate (in RIPA buffer) stained with ARG58777 anti-RFX5 antibody at 2 µg/ml and incubated for 1 hour at RT.
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ARG58777 anti-RFX5 antibody WB image
Western blot: 35 µg of NIH/3T3 cell lysate (in RIPA buffer) stained with ARG58777 anti-RFX5 antibody at 2 µg/ml and incubated for 1 hour at RT.
