ARG41278
anti-RFC4 antibody
anti-RFC4 antibody for ICC/IF,Immunoprecipitation,Western blot and Human,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes RFC4 |
|---|---|
| Tested Reactivity | Hu, Rat |
| Tested Application | ICC/IF, IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RFC4 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 204-363 of Human RFC4 (NP_002907.1). |
| Conjugation | Un-conjugated |
| Alternate Names | A1 37 kDa subunit; Activator 1 subunit 4; Replication factor C subunit 4; RFC37; Activator 1 37 kDa subunit; A1; RF-C 37 kDa subunit; Replication factor C 37 kDa subunit |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | HeLa | ||||||||
| Observed Size | 38 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | RFC4 |
| Gene Full Name | replication factor C (activator 1) 4, 37kDa |
| Background | The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008] |
| Function | The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit may be involved in the elongation of the multiprimed DNA template. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 40 kDa |
Images (3) Click the Picture to Zoom In
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ARG41278 anti-RFC4 antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG41278 anti-RFC4 antibody.
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ARG41278 anti-RFC4 antibody WB image
Western blot: 25 ug of HeLa cell lysate stained with ARG41278 anti-RFC4 antibody at 1:1000 dilution.
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ARG41278 anti-RFC4 antibody IP image
Immunoprecipitation: 200 ug extracts of K562 cells were immunoprecipitated and stained with ARG41278 anti-RFC4 antibody at 1:1000 dilution.
