ARG66457

anti-REG4 antibody

anti-REG4 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes REG4
Tested Reactivity Hu, Ms, Rat
Predict Reactivity Pig
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name REG4
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide within the center region of Human REG4.
Conjugation Un-conjugated
Alternate Names GISP; REG-like protein; Reg IV; RELP; Gastrointestinal secretory protein; Regenerating islet-derived protein 4; Regenerating islet-derived protein IV; REG-4; REG-IV

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100 - 1:200
WB1:500 - 1:1000
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol.
Preservative 0.01% Sodium azide
Stabilizer 30% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 445583 Rat REG4

GeneID: 67709 Mouse REG4

GeneID: 83998 Human REG4

Gene Symbol REG4
Gene Full Name regenerating islet-derived family, member 4
Function Calcium-independent lectin displaying mannose-binding specificity and able to maintain carbohydrate recognition activity in an acidic environment. May be involved in inflammatory and metaplastic responses of the gastrointestinal epithelium. [UniProt]
Cellular Localization Secreted. [UniProt]
Calculated MW 18 kDa

Images (2) Click the Picture to Zoom In

  • ARG66457 anti-REG4 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human brain. Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0). The section was then incubated with ARG66457 anti-REG4 antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • ARG66457 anti-REG4 antibody WB image

    Western blot: HEK293T, Raw264.7 and H9C2 whole cell lysates stained with ARG66457 anti-REG4 antibody.