ARG10619
anti-RBP4 antibody [RB42]
anti-RBP4 antibody [RB42] for ELISA,Western blot,Sandwich ELISA and Human
Overview
| Product Description | Mouse Monoclonal antibody [RB42] recognizes RBP4 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, WB, sELISA |
| Specificity | This antibody recognizes free RBP4 and RBP4 in complex with transthyretin. This antibody recognizes apo- as well as holo-RBP4. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | RB42 |
| Isotype | IgG1 |
| Target Name | RBP4 |
| Antigen Species | Human |
| Immunogen | Human plasma RBP4. |
| Conjugation | Un-conjugated |
| Alternate Names | Plasma retinol-binding protein; MCOPCB10; RDCCAS; RBP; Retinol-binding protein 4; PRBP |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4) and 0.1% Sodium azide |
| Preservative | 0.1% Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | RBP4 |
| Gene Full Name | retinol binding protein 4, plasma |
| Background | This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. [provided by RefSeq, Jul 2008] |
| Function | Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli. [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30283 RBP4 ELISA Antibody Duo Related products: RBP4 antibodies; RBP4 ELISA Kits; RBP4 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: A new role of fat – factory of circulating miRNAs |
| Calculated MW | 23 kDa |
Images (3) Click the Picture to Zoom In
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ARG10619 anti-RBP4 antibody [RB42] WB image
Western blot: Immunodetection of RBP4 in WB after SDS electrophoresis in reducing conditions by ARG10619 anti-RBP4 antibody [RB42]. 1 µg of purified endogenous RBP4 was loaded.
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ARG10619 anti-RBP4 antibody [RB42] sELISA image
Sandwich ELISA: Immunoreactivity of native free and native complexed RBP4 after several freeze-thaw cycles measured with the assay RB48 - RB42. Capture antibody: ARG10620 anti-RBP4 antibody [RB48] (1 µg/well); Detection antibody: ARG10619 anti-RBP4 antibody [RB42] labeled with stable Eu3+ chelate (0.2 µg/well). Antigen: Native isolated RBP4.
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ARG10619 anti-RBP4 antibody [RB42] sELISA image
Sandwich ELISA: Immunodetection of purified endogenous RBP4 in sandwich immunoassay by RB48-RB42 MAb assay in presence of 5 mM EDTA (blue line) or in absence of EDTA (pink line). Capture antibody: ARG10620 anti-RBP4 antibody [RB48] (1 µg/well); Detection antibody: ARG10619 anti-RBP4 antibody [RB42] labeled with stable Eu3+ chelate (0.2 µg/well). Antigen: Native isolated RBP4.
