ARG57093

anti-RBP4 antibody [2B4]

anti-RBP4 antibody [2B4] for Flow cytometry,Western blot and Human

Overview

Product Description Mouse Monoclonal antibody [2B4] recognizes RBP4
Tested Reactivity Hu
Tested Application FACS, WB
Host Mouse
Clonality Monoclonal
Clone 2B4
Isotype IgG1, kappa
Target Name RBP4
Antigen Species Human
Immunogen Recombinant fragment around aa. 19-201 of Human RBP4
Conjugation Un-conjugated
Alternate Names Plasma retinol-binding protein; MCOPCB10; 1-176; RDCCAS; RBP; Retinol-binding protein 4; PRBP; 1-182; 1-179; 1-181

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-dependent
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 10% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5950 Human RBP4

Swiss-port # P02753 Human Retinol-binding protein 4

Gene Symbol RBP4
Gene Full Name retinol binding protein 4, plasma
Background This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. [provided by RefSeq, Jul 2008]
Function Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli. [UniProt]
Calculated MW 23 kDa

Images (3) Click the Picture to Zoom In

  • ARG57093 anti-RBP4 antibody [2B4] FACS image

    Flow Cytometry: HepG2 cell line stained with ARG57093 anti-RBP4 antibody [2B4] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody: Mouse IgG (black line).

  • ARG57093 anti-RBP4 antibody [2B4] WB image

    Western blot: 60 µg of HepG2 cell lysate stained with ARG57093 anti-RBP4 antibody [2B4] at 1:500.

  • ARG57093 anti-RBP4 antibody [2B4] WB image

    Western blot: 10 µg of 1) 293T, and 2) RBP4 transfected 293T cell lysates stained with ARG57093 anti-RBP4 antibody [2B4] at 1:500.