ARG63745
anti-RBP1 antibody
anti-RBP1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Gene Regulation antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes RBP1 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Cow |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RBP1 |
| Antigen Species | Human |
| Immunogen | VEGVVCKQVFKKVQ |
| Conjugation | Un-conjugated |
| Alternate Names | RBPC; CRBP-I; Cellular retinol-binding protein I; CRBP; CRBP1; Cellular retinol-binding protein; CRABP-I; Retinol-binding protein 1; CRBPI |
Application Instructions
| Application Suggestion |
|
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|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene encodes the carrier protein involved in the transport of retinol (vitamin A alcohol) from the liver storage site to peripheral tissue. Vitamin A is a fat-soluble vitamin necessary for growth, reproduction, differentiation of epithelial tissues, and vision. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008] |
| Research Area | Gene Regulation antibody; Signaling Transduction antibody |
| Calculated MW | 16 kDa |
Images (5) Click the Picture to Zoom In
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ARG63745 anti-RBP1 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed U2OS cells permeabilized with 0.15% Triton. Cells were stained with ARG63745 anti-RBP1 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG63745 anti-RBP1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63745 anti-RBP1 antibody at 5 µg/ml dilution followed by AP-staining.
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ARG63745 anti-RBP1 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Pancreas. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63745 anti-RBP1 antibody at 5 µg/ml dilution followed by AP-staining.
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ARG63745 anti-RBP1 antibody WB image
Western blot: 35 µg of NIH/3T3 (A) and U251 (B) cell lysates (in RIPA buffer) stained with ARG63745 anti-RBP1 antibody at 2 µg/ml (A) and 0.5 µg/ml (B) dilutions and incubated at RT for 1 hour.
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ARG63745 anti-RBP1 antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed HeLa cells permeabilized with 0.5% Triton. Cells were stained with ARG63745 anti-RBP1 antibody (blue line) at 10 µg/ml dilution and overnight, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
