ARG43057
anti-RBFOX2 / RBM9 antibody
anti-RBFOX2 / RBM9 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes RBFOX2 / RBM9 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RBFOX2 / RBM9 |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to M1-Q81 of Human RBFOX2 / RBM9. |
| Conjugation | Un-conjugated |
| Alternate Names | RNA binding protein fox-1 homolog 2; dJ106I20.3; RTA; HRNBP2; HNRBP2; Fox-1 homolog B; RBM9; Hexaribonucleotide-binding protein 2; RNA-binding protein 9; fxh; Repressor of tamoxifen transcriptional activity; Fox-2; FOX2; RNA-binding motif protein 9 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | RBFOX2 |
| Gene Full Name | RNA binding protein, fox-1 homolog (C. elegans) 2 |
| Background | This gene is one of several human genes similar to the C. elegans gene Fox-1. This gene encodes an RNA binding protein that is thought to be a key regulator of alternative exon splicing in the nervous system and other cell types. The protein binds to a conserved UGCAUG element found downstream of many alternatively spliced exons and promotes inclusion of the alternative exon in mature transcripts. The protein also interacts with the estrogen receptor 1 transcription factor and regulates estrogen receptor 1 transcriptional activity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. Prevents binding of U2AF2 to the 3'-splice site. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis (By similarity). RNA-binding protein that seems to act as a coregulatory factor of ER-alpha. [UniProt] |
| Cellular Localization | Nucleus. Cytoplasm. [UniProt] |
| Calculated MW | 41 kDa |
Images (7) Click the Picture to Zoom In
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ARG43057 anti-RBFOX2 / RBM9 antibody ICC/IF image
Immunofluorescence: U2OS cells were blocked with 10% goat serum and then stained with ARG43057 anti-RBFOX2 / RBM9 antibody (green) at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG43057 anti-RBFOX2 / RBM9 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human melanoma tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43057 anti-RBFOX2 / RBM9 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43057 anti-RBFOX2 / RBM9 antibody WB image
Western blot: 50 µg of sample under reducing conditions. U2OS, Caco-2, A431, U-87MG, Rat brain and Mouse brain lysates stained with ARG43057 anti-RBFOX2 / RBM9 antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG43057 anti-RBFOX2 / RBM9 antibody FACS image
Flow Cytometry: SiHa cells were blocked with 10% normal goat serum and then stained with ARG43057 anti-RBFOX2 / RBM9 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG43057 anti-RBFOX2 / RBM9 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse gaster tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43057 anti-RBFOX2 / RBM9 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43057 anti-RBFOX2 / RBM9 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat gaster tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43057 anti-RBFOX2 / RBM9 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43057 anti-RBFOX2 / RBM9 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat gaster tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43057 anti-RBFOX2 / RBM9 antibody at 1 µg/ml dilution, overnight at 4°C.
