ARG59955
anti-RAP80 antibody
anti-RAP80 antibody for ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes RAP80 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RAP80 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-230 of Human RAP80 (NP_057374.3). |
| Conjugation | Un-conjugated |
| Alternate Names | RAP80; Retinoid X receptor-interacting protein 110; Receptor-associated protein 80; Ubiquitin interaction motif-containing protein 1; X2HRIP110; BRCA1-A complex subunit RAP80 |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | UIMC1 |
| Gene Full Name | ubiquitin interaction motif containing 1 |
| Function | Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1. [UniProt] |
| Cellular Localization | Nucleus. Note=Localizes at sites of DNA damage at double-strand breaks (DSBs). [UniProt] |
| Calculated MW | 80 kDa |
| PTM | Sumoylated. Phosphorylated upon DNA damage by ATM or ATR. [UniProt] |
Images (1) Click the Picture to Zoom In
-
ARG59955 anti-RAP80 antibody ICC/IF image
Immunofluorescence: GFP-RNF168 transgenic U2OS cells stained with ARG59955 anti-RAP80 antibody (Red). Green: GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining. GFP-RNF168 can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
