Overview

Product Description	Rabbit Polyclonal antibody recognizes RAP80
Tested Reactivity	Hu
Tested Application	IHC-P, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	RAP80
Antigen Species	Human
Immunogen	Synthetic peptide derived from Human RAP80.
Conjugation	Un-conjugated
Alternate Names	RAP80; Retinoid X receptor-interacting protein 110; Receptor-associated protein 80; Ubiquitin interaction motif-containing protein 1; X2HRIP110; BRCA1-A complex subunit RAP80

Application Instructions

Application Suggestion	Tested Application Dilution IHC-P 1:50 - 1:200 WB 1:1000 - 1:5000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	293T
Observed Size	~ 80 kDa

Properties

Form	Liquid
Purification	Affinity purified.
Buffer	PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 51720 Human UIMC1 Swiss-port # Q96RL1 Human BRCA1-A complex subunit RAP80
Gene Symbol	UIMC1
Gene Full Name	ubiquitin interaction motif containing 1
Function	Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1. [UniProt]
Cellular Localization	Nucleus. Note=Localizes at sites of DNA damage at double-strand breaks (DSBs). [UniProt]
Calculated MW	80 kDa
PTM	Sumoylated. Phosphorylated upon DNA damage by ATM or ATR. [UniProt]

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