ARG57715
anti-RAP1A + RAP1B antibody
anti-RAP1A + RAP1B antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes RAP1A + RAP1B |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RAP1A + RAP1B |
| Antigen Species | Human |
| Immunogen | Synthetic peptide from Human RAP1A. |
| Conjugation | Un-conjugated |
| Alternate Names | Ras-related protein Krev-1; C21KG; SMGP21; Ras-related protein Rap-1A; RAP1; KREV1; KREV-1; G-22K; GTP-binding protein smg p21A; K-REV; RAL1B |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Mouse lung | ||||||
| Observed Size | 21 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | RAP1A; RAP1B |
|---|---|
| Gene Full Name | RAP1A, member of RAS oncogene family; RAP1B, member of RAS oncogene family |
| Background | This gene encodes a member of the Ras family of small GTPases. The encoded protein undergoes a change in conformational state and activity, depending on whether it is bound to GTP or GDP. This protein is activated by several types of guanine nucleotide exchange factors (GEFs), and inactivated by two groups of GTPase-activating proteins (GAPs). The activation status of the encoded protein is therefore affected by the balance of intracellular levels of GEFs and GAPs. The encoded protein regulates signaling pathways that affect cell proliferation and adhesion, and may play a role in tumor malignancy. Pseudogenes of this gene have been defined on chromosomes 14 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014] |
| Function | Induces morphological reversion of a cell line transformed by a Ras oncogene. Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Together with ITGB1BP1, regulates KRIT1 localization to microtubules and membranes. Plays a role in nerve growth factor (NGF)-induced neurite outgrowth. Plays a role in the regulation of embryonic blood vessel formation. Involved in the establishment of basal endothelial barrier function. May be involved in the regulation of the vascular endothelial growth factor receptor KDR expression at endothelial cell-cell junctions. [UniProt] |
| Cellular Localization | Cell membrane, Lipid-anchor, Cytoplasm, perinuclear region, Cell junction, Early endosome. [UniProt] |
| Calculated MW | RAP1A and RAP1B: 21 kDa |
Images (1) Click the Picture to Zoom In
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ARG57715 anti-RAP1A + RAP1B antibody WB image
Western blot: 25 µg of Mouse lung lysate stained with ARG57715 anti-RAP1A + RAP1B antibody at 1:1000 dilution.
