ARG64141
anti-RAD9A antibody
anti-RAD9A antibody for Western blot and Human
Gene Regulation antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes RAD9A |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Dog |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RAD9A |
| Antigen Species | Human |
| Immunogen | C-QGPSPVLAEDSEGE |
| Conjugation | Un-conjugated |
| Alternate Names | DNA repair exonuclease rad9 homolog A; Cell cycle checkpoint control protein RAD9A; EC 3.1.11.2; RAD9; hRAD9 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q99638 Human Cell cycle checkpoint control protein RAD9A |
|---|---|
| Background | This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair. This protein possesses 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011] |
| Research Area | Gene Regulation antibody |
| Calculated MW | 43 kDa |
| PTM | Constitutively phosphorylated on serine and threonine amino acids in absence of DNA damage. Hyperphosphorylated by PRKCD and ABL1 upon DNA damage. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex. |
Images (1) Click the Picture to Zoom In
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ARG64141 anti-RAD9A antibody WB image
Western Blot: A431cell lysate (35 µg protein in RIPA buffer) stained with ARG64141 anti-RAD9A antibody at 0.3 µg/ml dilution.
