ARG45095
anti-RAD17 antibody
anti-RAD17 antibody for Western blot and S. pombe
Overview
| Product Description | Rabbit Polyclonal antibody recognizes RAD17 |
|---|---|
| Tested Reactivity | S. pombe |
| Tested Application | WB |
| Specificity | Reacts with the N-terminal region of S. pombe RAD17 protein |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RAD17 |
| Antigen Species | Yeast |
| Immunogen | Synthetic peptide corresponding to amino acids from the N-terminal region of S. pombe RAD17 protein |
| Conjugation | Un-conjugated |
| Alternate Names | RAD17; RAD17 Checkpoint Clamp Loader Component; RAD17Sp; Rad24; CCYC; Cell Cycle Checkpoint Protein RAD17; R24L; Cell Cycle Checkpoint Protein (RAD17); RAD17 Homolog (S. Pombe); RF-C Activator 1 Homolog; RF-C/Activator 1 Homolog; RAD1 (S. Pombe) Homolog; Rad17-Like Protein; RAD17 Homolog; RAD1 Homolog; HRAD17; HRad17 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Antigen Affinity Purified. |
| Buffer | PBS, 0.02% NaN3 |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | RAD17 |
|---|---|
| Background | The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified. [provided by RefSeq, Jul 2013] |
| Function | May also serve as a sensor of DNA replication progression. [Uniprot] |
| Cellular Localization | Chromosome, Nucleus. [Uniprot] |
| PTM | Phosphoprotein. [Uniprot] |
