ARG58009
anti-RACK1 / GBN2L1 antibody
anti-RACK1 / GBN2L1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Goat Polyclonal antibody recognizes RACK1 / GBN2L1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Cow, Dog |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RACK1 / GBN2L1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide from the internal region of Human RACK1 / GBN2L1 (NP_006089.1). (NTLGVCKYTVQDESH) |
| Conjugation | Un-conjugated |
| Alternate Names | HLC-7; H12.3; Gnb2-rs1; Cell proliferation-inducing gene 21 protein; Human lung cancer oncogene 7 protein; Guanine nucleotide-binding protein subunit beta-2-like 1; Receptor for activated C kinase; Guanine nucleotide-binding protein subunit beta-like protein 12.3; PIG21; Receptor of activated protein kinase C 1; RACK1 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 33 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Ammonium sulphate precipitation followed by affinity purification with immunogen. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | GNB2L1 |
| Gene Full Name | guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1 |
| Function | Involved in the recruitment, assembly and/or regulation of a variety of signaling molecules. Interacts with a wide variety of proteins and plays a role in many cellular processes. Component of the 40S ribosomal subunit involved in translational repression. Binds to and stabilizes activated protein kinase C (PKC), increasing PKC-mediated phosphorylation. May recruit activated PKC to the ribosome, leading to phosphorylation of EIF6. Inhibits the activity of SRC kinases including SRC, LCK and YES1. Inhibits cell growth by prolonging the G0/G1 phase of the cell cycle. Enhances phosphorylation of BMAL1 by PRKCA and inhibits transcriptional activity of the BMAL1-CLOCK heterodimer. Facilitates ligand-independent nuclear translocation of AR following PKC activation, represses AR transactivation activity and is required for phosphorylation of AR by SRC. Modulates IGF1R-dependent integrin signaling and promotes cell spreading and contact with the extracellular matrix. Involved in PKC-dependent translocation of ADAM12 to the cell membrane. Promotes the ubiquitination and proteasome-mediated degradation of proteins such as CLEC1B and HIF1A. Required for VANGL2 membrane localization, inhibits Wnt signaling, and regulates cellular polarization and oriented cell division during gastrulation. Required for PTK2/FAK1 phosphorylation and dephosphorylation. Regulates internalization of the muscarinic receptor CHRM2. Promotes apoptosis by increasing oligomerization of BAX and disrupting the interaction of BAX with the anti-apoptotic factor BCL2L. Inhibits TRPM6 channel activity. Regulates cell surface expression of some GPCRs such as TBXA2R. Plays a role in regulation of FLT1-mediated cell migration. Binds to Y.pseudotuberculosis yopK which leads to inhibition of phagocytosis and survival of bacteria following infection of host cells. Enhances phosphorylation of HIV-1 Nef by PKCs. Promotes migration of breast carcinoma cells by binding to and activating RHOA. [UniProt] |
| Calculated MW | 35 kDa |
| PTM | Phosphorylated on Tyr-228 and/or Tyr-246 by SRC. This is required for binding to SRC. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG58009 anti-RACK1 / GBN2L1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human small intestine tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG58009 anti-RACK1 / GBN2L1 antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG58009 anti-RACK1 / GBN2L1 antibody WB image
Western blot: 35 µg of Human lymph node, Mouse spleen and Rat spleen lysates (in RIPA buffer) stained with ARG58009 anti-RACK1 / GBN2L1 antibody at 0.1 µg/ml dilution. Primary incubation was 1 hour. Detected by chemiluminescence.
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ARG58009 anti-RACK1 / GBN2L1 antibody WB image
Western blot: 35 µg of NIH/3T3, HEK293, HeLa, A431, A549, MCF7, Jurkat and K562 cell lysates (in RIPA buffer) stained with ARG58009 anti-RACK1 / GBN2L1 antibody at 0.1 µg/ml dilution. Primary incubation was 1 hour. Detected by chemiluminescence.
